Rat IL-2/Interleukin-2 ELISA Kit PicoKine™

Rat IL-2/Interleukin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 22 publication(s).

Product Info Summary

SKU: EK0399
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Rat IL-2/Interleukin-2 ELISA Kit PicoKine™

See all IL-2 products

SKU/Catalog Number

EK0399

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-2/Interleukin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-2/Interleukin-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0399)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-Q155

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0399 is reactive to Il2 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0399 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0399-CAP Anti-Rat Il2 Pre-coated 96-well strip microplate 1
EK0399-ST Rat Il2 Standard 2 vials, 10 ng/tube
EK0399-DA Rat Il2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0240.0960.1730.3390.7641.0941.6962.295

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-2 in samples: 19219678 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-2/Interleukin-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)622431041562541022
Standard deviation4.7115.0653.094.8117.5271.54
CV (%)7.6%6.2%7%%8.6%6.9%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 162616063611.111.8%
Sample 224324727726725814.025.4%
Sample 3104199289788595365.236.8%
*number of samples for each test n=16.

Gene/Protein Information For Il2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17108

Gene ID

116562

Gene Name

Il2

Full Name

Interleukin-2

Weight

17.633kDa

Superfamily

IL-2 family

Alternative Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF Il2|interleukin 2|interleukin-2|IL-2|T-cell growth factor|TCGF

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il2, check out the Il2 Infographic

Il2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0399 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
Species: Rat
EK0399 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Immunomodulatory effect of Stichopus japonicus acid mucopolysaccharide on experimental hepatocellular carcinoma in rats

Suppression of complete Freund's adjuvant-induced adjuvant arthritis by cobratoxin

Increased secretion and expression of amylin in spontaneously diabetic Goto-Kakizaki rats treated with rhGLP-1 (7%u201336)

il-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line h446

Changes in the Interstitial Cells of Cajal and Immunity in Chronic Psychological Stress Rats and Therapeutic Effects of Acupuncture at the Zusanli Point (ST36)

Treatment of surgical brain injury by immune tolerance induced by intrathymic and hepatic portal vein injection of brain antigens

Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(?-amino ester) ester nanovector induces donor-specific liver allograft tolerance

Suppression of complete Freund's adjuvant-induced adjuvant arthritis by cobratoxin

Cheng K, Wu Z, Gao B, Xu J. Cell Biochem Biophys. 2014 Nov;70(2):1305-9. Doi: 10.1007/S12013-014-0055-9. Analysis Of Influence Of Baicalin Joint Resveratrol Retention Enema On The Tnf-??, Siga, Il-2, Ifn-?? Of Rats With Respiratory Syncytial Virus...

Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...

Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.

Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...

Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...

Liu Sh, Shen Xh, Wei Xf, Mao Xh, Huang T. Immunopharmacol Immunotoxicol. 2011 Mar;33(1):100-6. Doi: 10.3109/08923973.2010.483520. Epub 2010 May 20. Immunomodulatory Activity Of Butanol Extract From Solanum Lyratum In Tumor-Bearing Mice.

Eker Ss, Yavasci Eo, Cangur S, Kirli S, Sarandol E. Acta Neuropsychiatr. 2014 Oct;26(5):291-7. Doi: 10.1017/Neu.2014.13. Can Bdnf And Il-2 Be Indicators For The Diagnosis In Schizophrenic Patients With Depressive Symptoms?

Zhong G, Cheng X, Long H, He L, Qi W, Xiang T, Zhao Z, Zhu B. J Transl Med. 2013 Mar 21;11:71. Doi: 10.1186/1479-5876-11-71. Dynamically Expressed Microrna-15B Modulates The Activities Of Cd8+ T Lymphocytes In Mice With Lewis Lung Carcinoma.

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

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7 Customer Q&As for Rat IL-2/Interleukin-2 ELISA Kit PicoKine™

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-2 in Rat plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

V. Kaur

Verified customer

Asked: 2020-11-03

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-11-03

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-2 using the EK0399 Rat IL-2 Picokine® ELISA Kit?

P. Dhar

Verified customer

Asked: 2019-11-05

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-11-05

Question

Q: Can IL-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-03-13

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-03-13

Question

Q: how to proceed with the analysis of ELISA data? I have obtained IL-2 level in plasma.

M. Young

Verified customer

Asked: 2019-01-23

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-23

Question

Q: is it okay to use EDTA plasma as samples in Rat IL-2 Picokine® ELISA Kit (Catalog # EK0399)?

T. Turner

Verified customer

Asked: 2018-05-11

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-2 causing disruption of its protein structure. IL-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-2 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA, citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-11

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-04-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-04-26

Question

Dear Support: I want to purchase this product EK0399 but I want to know first if it can be used on tissue homogenates of rat. Please let me know. Thank you

Verified Customer

Verified customer

Asked: 2013-07-31

Answer

The product EK0399 kit has not been validated and tested with any lysate samples, however the kit is very accommodating to different samples types, so probably it will likely work on lysate. If you decided to work on this we recommend to dilute the sample at least 5 fold to minimize any effects of the detergents in the lysis buffer.

Boster Scientific Support

Answered: 2013-07-31

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