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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Rat RAGE ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat RAGE ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ager in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat RAGE ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0971)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q24-A342
78 pg/ml - 5,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0971 is reactive to Ager in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0971 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ager
RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
|EK0971-CAP||Anti-Rat Ager Pre-coated 96-well strip microplate||1|
|EK0971-ST||Rat Ager Standard||2 vials, 10 ng/tube|
|EK0971-DA||Rat Ager Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat RAGE PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat RAGE ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Ager in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ager (Source: Uniprot.Org, NCBI)
Advanced glycosylation end product-specific receptor
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1 Ager|RAGE|advanced glycosylation end product-specific receptor|advanced glycosylation end product-specific receptor|advanced glycosylation end product-specific receptor variant 2|advanced glycosylation end product-specific receptor variant 3|advanced glycosylation end product-specific receptor variant 4|advanced glycosylation end product-specific receptor variant 5|receptor for advanced glycosylation end products*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ager, check out the Ager Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ager: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0971 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protocatechuic acid improves hepatic insulin resistance and restores vascular oxidative status in type-2 diabetic rats
Cinnamaldehyde ameliorates STZ-induced rat diabetes through modulation of IRS1/PI3K/AKT2 pathway and AGEs/RAGE interaction
Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
EK0971 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Yudi Zhang,Chunhe Tao,Donglin Du, et al.Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway. Authorea. December 01, 2020. DOI: 10.22541/au.160684072.22355285/v1
EK0971 usage in article: APP:ELISA, SAMPLE:SERUM AND KIDNEY, DILUTION:NA
Liang Q,Lin Q,Li Y,Luo W,Huang X,Jiang Y,Qin C,Nong J,Chen X,Sooranna SR,Pinhu L.Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model.J Immunol Res.2020 Nov 25;2020:6644687.doi:10.1155/2020/6644687.PM
EK0971 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA
Radon-induced proteomic profile of lung tissue in rats
Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...
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