Rat RAGE ELISA Kit PicoKine™

Rat RAGE ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ager in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0971
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat RAGE ELISA Kit PicoKine™

See all Ager products

SKU/Catalog Number

EK0971

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat RAGE ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ager in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat RAGE ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0971)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-A342

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0971 is reactive to Ager in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0971 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0971-CAP Anti-Rat Ager Pre-coated 96-well strip microplate 1
EK0971-ST Rat Ager Standard 2 vials, 10 ng/tube
EK0971-DA Rat Ager Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0250.1150.2170.3680.6791.1291.5782.046

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat RAGE ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)13467730221357093219
Standard deviation7.3733.85211.548.7746.79267.17
CV (%)5.5%5%7%6.5%6.6%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ager in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11341381281491377.665.5%
Sample 26776696526636659.121.3%
Sample 33022291529842817293477.932.6%
*number of samples for each test n=16.

Gene/Protein Information For Ager (Source: Uniprot.Org, NCBI)

Uniprot ID

Q63495

Gene ID

81722

Gene Name

Ager

Full Name

Advanced glycosylation end product-specific receptor

Weight

42.664kDa

Alternative Names

advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1 Ager|RAGE|advanced glycosylation end product-specific receptor|advanced glycosylation end product-specific receptor|advanced glycosylation end product-specific receptor variant 2|advanced glycosylation end product-specific receptor variant 3|advanced glycosylation end product-specific receptor variant 4|advanced glycosylation end product-specific receptor variant 5|receptor for advanced glycosylation end products

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ager, check out the Ager Infographic

Ager infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ager: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0971 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
EK0971 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Yudi Zhang,Chunhe Tao,Donglin Du, et al.Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway. Authorea. December 01, 2020. DOI: 10.22541/au.160684072.22355285/v1
Species: Rat
EK0971 usage in article: APP:ELISA, SAMPLE:SERUM AND KIDNEY, DILUTION:NA

Liang Q,Lin Q,Li Y,Luo W,Huang X,Jiang Y,Qin C,Nong J,Chen X,Sooranna SR,Pinhu L.Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model.J Immunol Res.2020 Nov 25;2020:6644687.doi:10.1155/2020/6644687.PM
Species: Rat
EK0971 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Radon-induced proteomic profile of lung tissue in rats

Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...

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8 Customer Q&As for Rat RAGE ELISA Kit PicoKine™

Question

Q: how do I analyze ELISA data? I have obtained AGER level in cell lysates.

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-18

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of AGER in Rat cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

A. Puri

Verified customer

Asked: 2019-01-11

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the AGER ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-01-11

Question

Q: how to thaw whole blood sample for AGER ELISA after freezing?

P. Martinez

Verified customer

Asked: 2018-10-09

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test AGER for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-10-09

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-03-02

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-03-02

Question

Q: Can AGER ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-02-23

Question

Q: What is the optimal O.D. value for AGER ELISA kit? I used your AGER ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain AGER even though the O.D. values are not very high?

T. Mehta

Verified customer

Asked: 2017-04-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this AGER ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of AGER that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-04-12

Question

Q: can I use citrate plasma as samples in Rat AGER Picokine® ELISA Kit (Catalog # EK0971)?

Verified Customer

Verified customer

Asked: 2016-08-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of AGER causing disruption of its protein structure. AGER may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the AGER ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-08-08

Question

Q: if the enzyme conjugated AGER antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the AGER antigen?

Verified Customer

Verified customer

Asked: 2016-03-08

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-03-08

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