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Rat RANK ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat RANK. 96wells/kit, with removable strips.
Rat RANK ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK1281 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Rat |
| Sample Type: | cell culture supernates and serum. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 62.5pg/ml-4000pg/ml |
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Overview
| Product Name | Rat RANK ELISA Kit PicoKine™
See all primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK1281 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Rat Rank/TNFRSF11A with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Rank/TNFRSF11A tissue specificity: |
| Cite This Product | Rat RANK ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1281) |
Product Specs
| Reactivity/Species | Rat |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates and serum. |
| Sensitivity | <10pg/ml |
| Assay Range | 62.5pg/ml-4000pg/ml |
| Immunogen/Standard | Expression system for standard: NSO; Immunogen sequence: Q30-P213 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Gene/Protein Basic Information For Tnfrsf11a (Source: Uniprot.org, NCBI)
| Uniprot Id | F1M8Z6 |
|---|---|
| Gene Name | Tnfrsf11a |
| Molecular Weight | 67607 MW |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Tnfrsf11a (Source: Uniprot.org, NCBI)
| Background | Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda. |
|---|
Kit Components And Assay QC Data Details Of Rat RANK ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK1281-CAP | 96-well plate precoated with anti-Rat antibody | 1 |
| EK1281-ST | lyophilized recombinant Rat standard | 10ng/tube |
| EK1281-DA | biotinylated anti-Rat antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1281 Rat RANK ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.015 | 0.193 | 0.352 | 0.556 | 0.885 | 1.411 | 1.830 | 2.023 |
We measured random samples of Rat RANK ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 209 | 514 | 1401 | 226 | 542 | 1518 |
| Standard deviation | 13.58 | 34.95 | 88.16 | 18.3 | 42.81 | 97.15 |
| CV(%) | 6.5% | 6.8% | 7.9% | 8.1% | 7.9% | 6.4% |
We measured the performance consistency of Rat RANK ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 209 | 194 | 185 | 217 | 201 | 12.49 | 6.2% |
| Sample 2 | 514 | 489 | 548 | 539 | 522 | 23 | 4.4% |
| Sample 3 | 1401 | 1406 | 1464 | 1575 | 1461 | 70.05 | 4.7% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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| (888) 466-3604 |
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