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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin or EDTA)|
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Rat SCGB3A1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat SCGB3A1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat SCGB3A1 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SCGB3A1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1994)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: F22-G104
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1994 is reactive to SCGB3A1 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin or EDTA)
EK1994 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HIN-1/SCGB3A1
Secretoglobin family 3A member 1 is a protein that in humans is encoded by the SCGB3A1 gene. It is mapped to 10q21. Members of the secretoglobin family, such as SCGB3A1, are secreted proteins of about 10 kD that are found in high concentrations in fluids of lung, lacrimal gland, salivary gland, prostate, uterus, and other tissues.
|EK1994-CAP||Anti-Rat SCGB3A1 Pre-coated 96-well strip microplate||1|
|EK1994-ST||Rat SCGB3A1 Standard||2 vials, 10 ng/tube|
|EK1994-DA||Rat SCGB3A1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat SCGB3A1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect HIN-1/SCGB3A1, Dilution ratio of 1:1, concentration in serum is around 60pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SCGB3A1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of HIN-1/SCGB3A1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For SCGB3A1 (Source: Uniprot.Org, NCBI)
Secretoglobin family 3A member 1
cytokine high in normal-1; Cytokine HIN-1; High in normal 1; HIN1; HIN-1; HIN1Pneumo secretory protein 2; LU105; PNSP2; PnSP-2; PnSP-2Uteroglobin-related protein 2; SCGB3A1; secretoglobin family 3A member 1; secretoglobin, family 3A, member 1; UGRP2; UGRP2MGC87867 Scgb3a1|HIN, HIN-1, LuLe, LuLeu2, Pnsp2, UGR, UGRP2, Utgrp2|secretoglobin, family 3A, member 1|secretoglobin family 3A member 1|cytokine HIN-1|high in normal 1|pneumo secretory protein 2|uteroglobin related protein 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SCGB3A1, check out the SCGB3A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SCGB3A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK1994
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