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Product Info Summary
| SKU: | EK1559 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat TNFSF11/RANKL ELISA Kit PicoKine®
SKU/Catalog Number
EK1559
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat TNFSF11/RANKL ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tnfsf11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat TNFSF11/RANKL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1559)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: F141-D318
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1559 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1559 is reactive to Tnfsf11 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1559 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Tnfsf11
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. The rat gene is mapped to 15q11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1559-CAP | Anti-Rat Tnfsf11 Pre-coated 96-well strip microplate | 1 |
| EK1559-ST | Rat Tnfsf11 Standard | 2 vials, 10 ng/tube |
| EK1559-DA | Rat Tnfsf11 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.035 | 0.058 | 0.081 | 0.137 | 0.298 | 0.622 | 1.356 | 1.931 |
Data Example Images
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Rat TNFSF11/RANKL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Tnfsf11, Dilution ratio of 1:1, concentration in serum and plasma is 20-200 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat TNFSF11/RANKL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 37 | 209 | 464 | 38 | 208 | 445 |
| Standard deviation | 2.25 | 8.56 | 28.76 | 2.96 | 12.06 | 37.95 |
| CV (%) | 6.1% | 4.1% | 6.2% | 7.8% | 5.8% | 7.9% |
Specific Publications For Rat TNFSF11/RANKL ELISA Kit PicoKine® (EK1559)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Rat TNFSF11/RANKL ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TRANCE using the EK1559 Rat TRANCE Picokine® ELISA Kit?
K. Puri
Verified customer
Asked: 2020-11-30
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-30
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
C. Jackson
Verified customer
Asked: 2019-12-08
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-08
Question
I am looking for an anti-RANKL that is to be use for IHC-P on rat tissues, Is the product Rat TNFSF11/RANKL ELISA Kit PicoKine detects transmembrane forms of the protein?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The Rat TNFSF11/RANKL ELISA Kit PicoKine antibody does not carry on targeting the transmembrane portion of the protein and the sequence it target is outside aa 48-68.
Boster Scientific Support
Answered: 2019-05-29
Question
What is the recommended dilution ratio for serum samples for EK1559?
Verified customer
Asked: 2019-05-06
Answer
The Recommended dilution ratio for serum samples for the Rat TNFSF11/RANKL ELISA Kit PicoKine™ (EK1559) is 1:1.
Boster Scientific Support
Answered: 2019-05-07
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-03-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-03-16
Question
Q: What is the optimal O.D. value for TRANCE ELISA kit? I performed your TRANCE ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TRANCE even though the O.D. values are not very high?
K. Harris
Verified customer
Asked: 2018-09-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TRANCE ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TRANCE that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-09-23
Question
Q: can I use citrate plasma as samples in Rat TRANCE Picokine® ELISA Kit (Catalog # EK1559)?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TRANCE causing disruption of its protein structure. TRANCE may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TRANCE ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-17
Question
A customer wanted to know the recommended dilution ratio for serum samples for EK1559.
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
Recommended dilution ratio for serum samples for EK1559 is 1:1.
Boster Scientific Support
Answered: 2017-06-06
Question
Q: Can TRANCE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2014-09-02
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2014-09-02
Question
My standard curve values are really high. I was wondering if the antibody diluent caused increased background. The lot no. is 11001516121. i attach the data. Columns 1 and 2 are standard curve duplicates and 3, 4 are my serum samples.
Verified Customer
Verified customer
Asked: 2013-11-25
Answer
Please suggest the customer repeat the experiment by adhering to our guides. 1. Shorten the incubation time of Color Developing Reagent (TMB). 2.Confirm that wash apparatus is working correctly and each well is throughly washed but not dry. Or follow the instructions stated on the datasheet "Add 300
Boster Scientific Support
Answered: 2013-11-25
