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- Table of Contents
for Your Multiplex IF Needs
Custom Multi-Host Polyclonal Antibody Production Services
Multi-host antibody development designed for high-specificity multiplex immunofluorescence panels—affordable, flexible, and validated.
Building a multiplex immunofluorescence (IF) panel often fails at one step: finding antibodies for every marker in compatible host species. Boster Bio offers custom multi-host polyclonal antibody production services —beyond rabbit and mouse—so you can complete complex, multi-color IF panels with the right host strategy.
From antigen design to affinity purification, we track ELISA titers and antigen binding at key milestones to help ensure consistent performance and predictable QC deliverables.
Begin Inquiry
Expand Your Multiplex IF Panel with More Host Options
Quick selection guide: Decide your host strategy → Select a plan (Fast / Standard / Co-development) → Share your target + application to receive a quote.
Start from your panel—not from what’s available in rabbit or mouse. Our multi-host polyclonal service helps you complete multiplex IF panels by selecting the best host species for each marker and reducing secondary cross-reactivity risks.
If rabbit is already the right fit for your rare-species target, our $600 rabbit antibody production service can be a fast starting point.
In addition to rabbit immunization, we support alternative species and can adapt immunization schedules based on host-specific factors.
What you get: affinity-purified polyclonal antibody (tier-based amount) + ELISA titer report + COA.
Optional add-ons: IF/WB/IHC/FC validation using your samples or our in-house tissue library.
Co-development
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 2 rabbits
- 4 immunizations per rabbit
- 1 serum collection per rabbit
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 100μg affinity purified antibody
$800
Standard
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 2 rabbits
- 4 immunizations per rabbit
- 1 serum collection per rabbit
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 3-5mg affinity purified antibody
$1300
Fast
Time: 45 days
Antigen Preparation
- Client Provided Antigen
- Boster Provide Antigen (+5 weeks)
Immunization (5 weeks)
- 2 rabbits
- 2 immunizations per rabbit
- 1 serum collection after the 2nd shot
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 2nd shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 3-5mg affinity purified antibody
Boost Immunization & Final Purification
Boost Immunization(4 weeks)
- 3rd and 4th immunization per rabbit 2nd serum collection after the 4th shot.
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot.
- ELISA testing of the antibody titer against the antigen.
Deliverables
- COA report
- 3-5mg affinity purified antibody
$1300
Co-development
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 1 chicken
- 4 immunizations per chicken
- 1 egg collection per chicken
Purification and Testing (1 week)
- Zinc sulfate precipitation of yolk after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 1mg IgY (Zinc Sulfate Precipitation)
$800
Standard
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 1 chicken
- 4 immunizations per chicken
- 1 egg collection per chicken
Purification and Testing (1 week)
- Zinc sulfate precipitation of yolk after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 20-50mg IgY (Zinc Sulfate Precipitation)
$1300
Fast
Time: 45 days
Antigen Preparation
- Client Provided Antigen
- Boster Provide Antigen (+5 weeks)
Immunization (5 weeks)
- 1 chicken
- 2 immunizations per chicken
- 1 egg collection after the 2nd shot
Purification and Testing (1 week)
- Zinc sulfate precipitation of yolk after the 2nd shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 20-50mg IgY(Zinc Sulfate Precipitation)
Boost Immunization & Final Purification
Boost Immunization (4 weeks)
- 3rd and 4th immunization per chicken 2nd egg collection after the 4th shot.
Purification and Testing (1 week)
- Zinc sulfate precipitation of yolk after the 4th shot.
- ELISA testing of the antibody titer against the antigen.
Deliverables
- COA report
- 20-50mg IgY (Zinc Sulfate Precipitation)
$1300
Co-development
Time: 136 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (13 weeks)
- 1 goat
- 4 immunizations per goat
- 1 serum collection per goat
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 5mg affinity purified antibody
$2800
Standard
Time: 136 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (13 weeks)
- 1 goat
- 4 immunizations per goat
- 1 serum collection per goat
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 50-100mg affinity purified antibody
$3600
Fast
Time: 59 days
Antigen Preparation
- Client Provided Antigen
- Boster Provide Antigen (+5 weeks)
Immunization (7 weeks)
- 1 goats
- 2 immunizations per goat
- 1 serum collection after the 2nd shot
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 2nd shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 50-100mg affinity purified antibody
Boost Immunization & Final Purification
Boost Immunization(6 weeks)
- 3rd and 4th immunization per goat 2nd serum collection after the 4th shot.
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot.
- ELISA testing of the antibody titer against the antigen.
Deliverables
- COA report
- 50-100mg affinity purified antibody
$3600
Co-development
Time: 136 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (13 weeks)
- 1 alpaca
- 4 immunizations per alpaca
- 1 serum collection per alpaca
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 5mg affinity purified antibody
$6300
Standard
Time: 136 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (13 weeks)
- 1 alpaca
- 4 immunizations per alpaca
- 1 serum collection per alpaca
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 50-100mg affinity purified antibody
$7500
Fast
Time: 59 days
Antigen Preparation
- Client Provided Antigen
- Boster Provide Antigen (+5 weeks)
Immunization (7 weeks)
- 1 alpacas
- 2 immunizations per alpaca
- 1 serum collection after the 2nd shot
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 2nd shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 50-100mg affinity purified antibody
Boost Immunization & Final Purification
Boost Immunization(6 weeks)
- 3rd and 4th immunization per alpaca 2nd serum collection after the 3rd shot.
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot.
- ELISA testing of the antibody titer against the antigen.
Deliverables
- COA report
- 50-100mg affinity purified antibody
$7500
Co-development
Time: 136 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (13 weeks)
- 1 donkey
- 4 immunizations per donkey
- 1 serum collection per donkey
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 5mg affinity purified antibody
$5200
Standard
Time: 136 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (13 weeks)
- 1 donkey
- 4 immunizations per donkey
- 1 serum collection per donkey
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 50-100mg affinity purified antibody
$6300
Fast
Time: 59 days
Antigen Preparation
- Client Provided Antigen
- Boster Provide Antigen (+5 weeks)
Immunization (7 weeks)
- 1 donkey
- 2 immunizations per donkey
- 1 serum collection after the 2nd shot
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 2nd shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 50-100mg affinity purified antibody
Boost Immunization & Final Purification
Boost Immunization(6 weeks)
- 3rd and 4th immunization per donkey 2nd serum collection after the 3rd shot.
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot.
- ELISA testing of the antibody titer against the antigen.
Deliverables
- COA report
- 50-100mg affinity purified antibody
$6300
Co-development
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 5 guinea pigs
- 4 immunizations per guinea pig
- 1 serum collection per guinea pig
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 100μg affinity purified antibody
$1300
Standard
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 5 guinea pigs
- 4 immunizations per guinea pig
- 1 serum collection per guinea pig
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 5mg affinity purified antibody
$1850
Co-development
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 5 mice
- 4 immunizations per mouse
- 1 serum collection per mouse
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 100μg affinity purified antibody
$1700
Standard
Time: 108 days
Antigen Preparation (5 weeks)
- Antigen Design
- Peptide or Recombinant Protein
Immunization (9 weeks)
- 5 mice
- 4 immunizations per mouse
- 1 serum collection per mouse
Purification and Testing (1 week)
- Antigen affinity purification of serum after the 4th shot
- ELISA testing of the antibody titer against the antigen
Deliverables
- COA report
- 1mg affinity purified antibody
$2250
Antigen Design Tailored for
Immunofluorescence (IF)
We don’t just synthesize antigens—we design recombinant protein antigens to perform under multiple conditions.
We also offer custom antigen formats, including modified proteins, to improve
immunogenicity or mimic post-translational states
relevant to your target.
All antigen production and protein expression are handled in-house to maintain strict quality standards throughout the workflow.
Want to see how it works? We can walk you through the science behind our antigen design process using detailed project briefings
or a custom video overview. For downstream purification, Protein A columns may be used depending on the antibody isotype and host species.
When applicable, Protein G is used for affinity purification, particularly for subclasses that bind poorly to Protein A.
Optional IF Validation Services
Unlike most vendors, we don’t leave you guessing post-purification.
We offer optional immunofluorescence validation using your own samples or tissue types from our in-house library.
This means less time spent troubleshooting and more confidence in your polyclonal antibodies.
We also provide ELISA titers for tracking antibody responses and can support Fc fragment analysis as needed.
Shared Development, Lower Cost
If your target is part of a high-interest pathway or disease model, our co-development model can significantly reduce your cost. We retain the right to commercialize the antibody after your project, and in return, you receive reduced pricing on development and full use of polyclonal antibodies for your work.
What Makes Our Multi-Host Custom
Antibody Programs Unique?
Built for multiplex immunofluorescence workflows—so you can finish your panel with the right host options, predictable QC, and optional application validation.
Antibody production TIMELINE
Below is a general timeline of a custom polyclonal antibody project across various host species. For large animals (such as goats, alpacas, and donkeys), we extend each immunization cycle by one week to ensure the effectiveness of the immunization. For any questions, please contact support@bosterbio.com
| Milestone | Start Day | Description | Deliverables |
|---|---|---|---|
| Pre-immune sera collection (optional) | Day 0 | (Optional) Pre-immune bleed ~0.5ml per host animal. | |
| Plasmid synthesis | Day 1 | Synthesizing your antigen expression plasmid. | |
| Recombinant antigen expression | Day 14 | Express your protein antigen recombinantly. | (Optional) Pre-immune screening report |
| Immunization preparation | Day 35 (Day 1 if you provide antigen) | Preparing antigen with CFA or IFA, selecting and preparing the chosen host species. | |
| Immunization 1st shot | Day 52 | 2 host animals (e.g., rabbit, guinea pig, chicken, or goat), 8-10 subcutaneous immunizations, ~0.5mg of antigen total per animal. | |
| Immunization 2nd shot | Day 73 | 6-8 subcutaneous immunizations, ~0.25mg of antigen total per animal. | |
| Immunization 3rd shot | Day 87 | 6-8 subcutaneous immunizations, ~0.25mg of antigen total per animal. | |
| Immunization 4th shot | Day 101 | 6-8 subcutaneous immunizations, ~0.25mg of antigen total per animal. | |
| Sera collection, purification and ELISA test | Day 115 | Purify the antisera with antigen affinity chromatography, perform ELISA testing, and decide whether to continue or terminate the project based on ELISA results and (optional) specific application testing. | (Optional) A sample vial of antibody in a custom amount for specific application validation. |
| Prepare antibody for shipping | Day 108 | Prepare antibodies for shipping, which may take 10-15 days. Deliver the final report to the customer online. |
|
What You’ll Receive
- Affinity-purified polyclonal antibody (100–500 µg, depending on service tier)
- Optional pre-immune serum
- ELISA test report
- Certificate of Analysis (COA)
- Optional validation sample for application-specific testing
- Recombinant expression and QC report of the target antigen (if applicable)
Ready to Start?
Submit your project requirements today and our scientists will provide a formal quote and consultation within 1 business day.
Request a quote
Frequently Asked Questions
What do I need to start a project?
Just send us your target sequence or peptide info. We’ll handle everything from antigen design to antibody purification.
What host species are available?
We offer rabbit, goat, chicken, guinea pig, and alpaca. Our team can recommend the most suitable host based on your target and intended application. For each host, we adjust the immunization schedules to optimise immune response and yield.
Can I include IF validation in my order?
Yes. Immunofluorescence validation is available as an add-on. We’ll test the antibody in your samples or using tissue from our internal collection. For certain projects, ELISA kits may also be used for quantifying binding performance.
How long does the full process take?
Most projects take around 122 days. We’ll provide milestone updates throughout and can advise on urgent requests.
How much antibody will I receive?
Standard yields range from 100–500 µg of affinity-purified antibody, depending on the plan. Larger quantities are available upon request.