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Product Info Summary
|Description:||Transient overexpression lysate of TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2)|
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TAF2 (NM_003184) Human Over-expression Lysate
Transient overexpression lysate of TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2)
Storage & Handling
Cite This Product
TAF2 (NM_003184) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006873)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TAF2 Human Over-expression Lysates NM_003184.
Western blot validation of overexpression lysate (LS006873) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TAF2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TAF2 (Source: Uniprot.org, NCBI)
Transcription initiation factor TFIID subunit 2
150 kDa cofactor of initiator function; CIF150TAFII-150; cofactor of initiator function, 150kD subunit; TAF(II)150; TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF2B; TAFII150RNA polymerase II TBP-associated factor subunit B; TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD; TBP-associated factor 150 kDa; Transcription initiation factor TFIID 150 kDa subunit; transcription initiation factor TFIID subunit 2 TAF2 CIF150, MRT40B, TAFII150, TAF2 TATA-box binding protein associated factor 2 transcription initiation factor TFIID subunit 2|150 kDa cofactor of initiator function|RNA polymerase II TBP-associated factor subunit B|TAF(II)150|TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa|TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD|TBP-associated factor 150 kDa|cofactor of initiator function, 150kD subunit|transcription initiation factor TFIID 150 kDa subunit*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on TAF2, check out the TAF2 Infographic
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No publications found for LS006873
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