Product Info Summary
SKU: | M30971 |
---|---|
Size: | 100μl/vial |
Reactive Species: | All species |
Host: | Mouse |
Application: | Dot blot, ELISA, WB |
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Product info
Product Name
Anti-Flag Tag Antibody (monoclonal, 1E6)
SKU/Catalog Number
M30971
Size
100μl/vial
Form
Liquid
Description
Boster Bio Anti-Flag Tag Antibody (monoclonal, 1E6) catalog # M30971. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flag Tag Antibody (monoclonal, 1E6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30971)
Host
Mouse
Contents
0.5mg/ml in PBS, pH7.4, containing 50% glycerol.
Clonality
Monoclonal
Clone Number
1E6
Isotype
IgG2b
Immunogen
This antibody is produced by immunizing animals with a synthetic Flag tag peptide corresponding to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M30971 is reactive to in All species
Applications
M30971 is guaranteed for Dot blot, ELISA, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
Background of
FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
1:500-1:5000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of different concentration of Anti-Flag (1E6) Monoclonal Antibody
Antigen: Extracts from E.coli expressing Flag-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins
Specific Publications For Anti-Flag Tag Antibody (monoclonal, 1E6) (M30971)
Hello CJ!
M30971 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Clinical and prognostic role of annexin A2 in adamantinomatous craniopharyngioma
Gan RH,Lin LS,Zheng DP,Zhao Y,Ding LC,Zheng DL,Lu YG.High expression of Notch2 drives tongue squamous cell carcinoma carcinogenesis.Exp Cell Res.2020 Dec 28;399(1):112452.doi:10.1016/j. yexcr.2020.112452. Epub ahead of print.PMID:33382997.
Species: Human,Mouse
M30971 usage in article: APP:WB, SAMPLE:CAL-27 CELL, DILUTION:1:500
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