Anti-Flag Tag Antibody (monoclonal, 1E6)

Product Name

Anti-Flag Tag Antibody (monoclonal, 1E6)

View all Tmem177 Antibodies

SKU/Catalog Number

M30971

Size

100μl/vial

Form

Liquid

Description

Boster Bio Anti-Flag Tag Antibody (monoclonal, 1E6) catalog # M30971. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Flag Tag Antibody (monoclonal, 1E6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30971)

Host

Mouse

Contents

0.5mg/ml in PBS, pH7.4, containing 50% glycerol.

Clonality

Monoclonal

Clone Number

1E6

Isotype

IgG2b

Immunogen

This antibody is produced by immunizing animals with a synthetic Flag tag peptide corresponding to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M30971 is reactive to Tmem177 in All species

Applications

M30971 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

34.067kDa

Background of Tmem177

FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

1:500-1:5000

Validation Images & Assay Conditions

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Western blot analysis of different concentration of Anti-Flag (1E6) Monoclonal Antibody
Antigen: Extracts from E.coli expressing Flag-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins

Gene/Protein Information For Tmem177 (Source: Uniprot.org, NCBI)

Alternative Names

MGC10993; transmembrane protein 177

For more info on Tmem177, check out the Tmem177 Infographic

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In this infographic, you will see the following information for Tmem177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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