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The antibody shows a clear target band, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU P00003
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, total MTOR protein levels were similar across all groups. However, phosphorylated MTOR levels showed significant changes: the blank group was the lowest, the model group the highest, and in the drug-treated groups, phosphorylation decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

The antibody shows a clear target, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU P00024-4
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-AKT1,2,3 Antibody Picoband®
Primary Incubation 1:10000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary Under equal loading conditions, the total AKT protein levels were similar across all groups, while the amount of phosphorylated AKT1 showed significant changes. The blank group had the lowest level, the model group had the highest, and in the drug-treated groups, the levels decreased progressively from low to medium to high dose. The positive control drug group was close to the high-dose group.

The antibody shows a clear target, correct localization, minimal background, and overall good results.

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Anti-ATF4 Rabbit Monoclonal Antibody
Primary Incubation 1:10000, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, the total MTOR protein levels were similar across all groups. However, the levels of phosphorylated MTOR showed significant changes: the blank group had the lowest, the model group the highest, and in the drug-treated groups, the phosphorylation levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

Boster antibodies have good quality and high cost-effectiveness, and will be my preferred brand in the future.

Excellent, submitted by on
SKU A00024-2
Application Western Blot
Sample mouse 4T1 cells
Sample Processing Description normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Primary Incubation 1:2500, overnight at 4 ℃
Secondary Antibody HRP Goat Anti-Rabbit IgG
Secondary Incubation 1:10000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary With equal loading amounts, the total AKT protein levels were similar across all groups. However, the levels of phosphorylated AKT1 showed significant changes: the blank group had the lowest, the model group the highest, and in the drug-treated groups, phosphorylation levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

Our lab will continue to use this antibody, and we will recommend it to other researchers at the university working on similar studies.

Excellent, submitted by on
SKU A31732-2
Application Immunofluorescence
Sample Normal rat liver and alcoholic rat liver
Sample Processing Description The samples consist of myocardium from normal SD rats and myocardium from SD rats subjected to an alcoholic liver disease model. The alcoholic liver disease model was established by gavaging 50% ethanol twice daily, while allowing the rats to freely consume alcoholic beverages, for a total of 14 weeks.
Other Reagents Goat Serum, DAPI, Anti-fade mounting medium
Primary Antibody Anti-PTRF/CAVIN1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 488 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:500, 45 minutes in room temperature
Detection Imaging system:Leica DM2500
Results Summary PTRF/CAVIN1 is a multifunctional protein that shuttles between the plasma membrane and the nucleus, assisting in the recruitment of repair proteins when the plasma membrane is damaged. The results of this IF experiment indicate that alcohol gavage induces myocardial injury and leads to increased expression of PTRF.

This antibody has good specificity, clearly labels endothelial cells, exhibits low background, and produces very clean and beautiful results.

Excellent, submitted by on
SKU A01513-3
Application Immunofluorescence
Sample Normal rat liver and alcoholic rat liver
Sample Processing Description The samples consist of livers from normal SD rats and livers from SD rats with an alcoholic liver disease (ALD) model. The ALD model was established by gavaging the rats twice daily with 50% alcohol, while allowing them free access to alcoholic beverages, for a continuous period of 14 weeks.
Other Reagents Goat Serum, DAPI, Anti-fade mounting medium
Primary Antibody Anti-CD31/Pecam1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)
Secondary Incubation 1:500, 45 minutes in room temperature
Detection Imaging system:Leica DM2500
Results Summary CD31 is a marker of vascular endothelial cells. In normal liver, it is expressed in the endothelium of blood vessels, while liver sinusoidal endothelial cells (LSECs) show minimal expression. However, in alcoholic liver disease, the phenotype of LSECs changes and they begin to express CD31, leading to an overall increase in CD31 expression in the liver. This has also been confirmed by experimental results.

Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of

Excellent, submitted by on
SKU M00003
Application Western Blot
Sample human OCI-LY1 cell
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

The CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear with no nonspecific signals. After recovery and reuse, the antibody still performed well.

Excellent, submitted by on
SKU PB9233
Application Western Blot
Sample mouse uterine tissue
Sample Processing Description Mouse uterine tissues were minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody Transferrin Receptor/TFRC Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:Tanon
Results Summary The CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear with no nonspecific signals, and after recovery and reuse, the antibody still showed good performance.

This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has well-defined edges.

Excellent, submitted by on
SKU P00003
Application Western Blot
Sample human OCI-LY1 cell
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.

Excellent, submitted by on
SKU M00220-1
Application Western Blot
Sample mouse lung cancer tissue
Sample Processing Description Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Twenty microliters of each protein sample were loaded per lane onto an SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody CD86/B7 2 Rabbit Monoclonal Antibody
Primary Incubation 1:2000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL reagent, Imaging system:Tanon
Results Summary This antibody is highly specific and efficient, with a clear target band and no nonspecific bands.