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Anti-C-Terminal CD44 Antibody for Western Blotting

Excellent, submitted by Elena Deryugina on 2024-07-25

Source: Biocompare.com

SKU	PA1021-2
Application	Western Blot
Sample	Tumor cell lysate
Primary Incubation	Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw
Blocking Agent	5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Secondary Incubation	Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw
Tertiary Incubation	HRP-bound secondary antibodies were detected by WestPico from ThermoScientific/Tw
Detection	Chemiluminescence: West Pico from Thermo Scientific
Results Summary	The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20℃.

"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane."

A Nice Antibody For Gp91phox Subunit Of NOX System

Excellent, submitted by Rahul Kumar on 2024-07-25

Source: Biocompare.com

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SKU	PA1667
Application	Western Blot
Sample	22RV1 cytosolic and membrane fractions
Primary Incubation	1:1000, overnight at 4 degree
Blocking Agent	5% Milk
Secondary Incubation	1:5000, 1 hour RT.
Detection	ECL
Results Summary	Please refer our paper (Scientific Reports 6, Article number: 23135 (2016)doi:10.1038/srep23135).

"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."

Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody

Excellent, submitted by Bastien Moës on 2024-07-16

Source: Biocompare.com

SKU	P01424-1
Application	Flow Cytometry
Sample	B cells
Primary Incubation	0.25 µl/10^6 cells 50min RT
Blocking Agent	FBS 5%
Secondary Incubation	F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6
Detection	Flow Cytometry
Results Summary	Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.

Anti-ULK2 Antibody Works Great for Western Blotting

Excellent, submitted by Bence Hajdú on 2024-07-15

Source: Biocompare.com

SKU	A05219
Application	Western Blot
Sample	HEK293T
Primary Incubation	1:5000 for 24hours at 4degrees celsius.
Blocking Agent	Skim milk
Secondary Incubation	1 hour room temperature 1:3000
Detection	ECL
Results Summary	We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins.

"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."

The antibody worked well even though we used fresh frozen brain sections cut at 20 micron thickness thaw-mounted onto microscope slide that were then post-fixed with 4% paraformaldehyde. The antibody worked equally well when used on fixed brains sectioned

Excellent, submitted by Bob Speth from Nova Southeastern University on 2024-06-19

Boster bio GFAP 1:100 PFA (7-2) - cortex 12x - with scale bar

Boster bio GFAP 1:100 PFA (7-2) - cortex 20x - with scale bar

Boster bio GFAP 1:250 12x (free-floating)

SKU	PB9082
Application	Immunofluorescence
Sample	Mouse brain
Primary Antibody Dilution	1:100

Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of GFAP (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.

Immunohistochemistry was also performed with free-floating sections, which were exposed to the same primary antibodies diluted (1:250) in blocking buffer overnight and, subsequently, exposed to secondary antibodies diluted in blocking buffer for 1 hour, following the same procedure as the mounted sections. After incubation, sections were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse and transferred with a painting brush to a container filled with 1X PBS with 0.01% sodium azide, from which they were mounted onto slides. Slides were left to dry in the dark, after which mounting media was added to cover slip the sections. As with slide-mounted sections, these slides were kept in the dark at 4 ˚C prior to imaging.

Immunohistochemistry for Anti-CD31/PECAM1 Antibody

Excellent, submitted by Z. Edwards on 2020-08-06

SKU	M01513-4
Application	Immunohistochemistry (Paraffin-embedded)
Blocking step	5% BSA as a blocking agent for 30 min at 37°C
Sample	Human Lung
Fixative	Fixed with 4% paraformaldehyde
Primary Ab Incubation	37°C for 30 minutes
Primary Ab Incubation diluent	5% BSA in TBS
Primary Ab Concentration	1ug/ml
Secondary Antibody	SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution	The kit was ready to use, no dilution needed
Secondary Ab Incubation	at 37°C for 30 min

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by Xiangrong Geng on 2020-07-30

Source: Biocompare.com

SKU	M00593
Application	Western Blot
Sample	HEK293T
Detection	ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Immunocytochemistry for Anti-E Cadherin 1/CDH1 Antibody

Excellent, submitted by E.Hernandez on 2020-07-18

The antibody worked perfectly, Great sensitivity

SKU	PB9561
Application	Immunohistochemistry (paraffin-embedded)
Blocking step	5% BSA as a blocking agent for 30 min at 37°C
Sample	Mouse Liver
Fixative	Fixed with 4% paraformaldehyde
Primary Ab Incubation	4°C overnight
Primary Ab Incubation diluent	5% BSA in TBS
Primary Ab Concentration	2ug/ml
Secondary Antibody	SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution	The kit was ready to use, no dilution needed
Secondary Ab Incubation	4°C overnight

Immunohistochemistry for Anti-E Cadherin 1 CDH1 Antibody

Excellent, submitted by K. Evans on 2020-07-16

SKU	M00063-2
Application	Immunohistochemistry (Paraffin-embedded)
Blocking step	5% BSA as a blocking agent for 30 min at 37°C
Sample	Human Oesophagus
Fixative	Fixed with 4% paraformaldehyde
Primary Ab Incubation	4°C overnight
Primary Ab Incubation diluent	5% BSA in TBS
Primary Ab Concentration	2ug/ml
Secondary Antibody	SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution	The kit was ready to use, no dilution needed
Secondary Ab Incubation	at 37°C for 30 min

Great PKC antibody for IHC

Excellent, submitted by C Parson on 2020-03-23

We used Anti-PKC Alpha Picoband™ Antibody (SKU: PB9318) for IHC experiment from mouse intestine tissues. Clear positive staining with the right subcellular locations. Super easy to determine the dilution ratio! Great quality.