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WB analysis using Anti-EGFR antibody (M00023-2) in mouse hippocampal tissue showed a clear band at the expected molecular weight with low background, demonstrating good specificity and reliable performance.

Excellent, submitted by Yajun Qiao, Qinghai University on 2026-05-08

SKU	M00023-2
Application	Western Blot
Sample	mouse hippocampal tissue
Sample Processing Description	The left hippocampus was dissected from normal mouse brains, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	EGFR (ErbB 1) Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	EGFR protein is a key molecular switch located on the cell membrane that receives extracellular signals and drives cell proliferation and differentiation through kinase activity. It serves as a central regulator of organ development, tissue homeostasis, and regenerative repair. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the BDNF antibody. The results showed a clear and correctly positioned target band, indicating that the antibody is functional and works properly.

WB analysis using Anti-CDK2 antibody (M00166) in mouse hippocampal tissue showed a specific band at the expected size with low background, indicating good performance.

Excellent, submitted by Yajun Qiao, Qinghai University on 2026-05-06

SKU	M00166
Application	Western Blot
Sample	mouse hippocampal tissue
Sample Processing Description	Total protein was extracted from the left hippocampus of normal mouse brain.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	CDK2 Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	CDK2 (cyclin-dependent kinase 2) plays a central role in driving the cell cycle and serves as a key node integrating proliferative signaling, DNA damage response, and chemotherapy stress. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the CDK2 antibody. The results showed a specific band at the expected molecular weight with good signal quality, indicating reliable antibody performance.

WB analysis using Anti-ATP1A1 antibody (M00956) in HepG2 cells showed a clear and specific band at the expected molecular weight, with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by Yumeng Han, Nanjing Medical University on 2026-05-06

SKU	M00956
Application	Western Blot
Sample	human adrenocortical carcinoma tissue
Sample Processing Description	Total protein was extracted from clinically collected human adrenocortical carcinoma tissue.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	Sodium Potassium ATPase ATP1A1 Rabbit Monoclonal Antibody
Primary Incubation	1:5000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1h in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	ATP1A1 encodes the α1 catalytic subunit of the Na⁺/K⁺-ATPase, whose core physiological function is to maintain the transmembrane sodium and potassium electrochemical gradient, serving as the basis for nearly all vital processes such as neuronal excitability, renal reabsorption, and muscle contraction. In this study, normally cultured HepG2 cells (without any drug treatment) were used to validate the quality of the ATP1A1 antibody. The results showed a clear target band at the correct position with clean background, indicating that the antibody performs well.

The Anti-FOXO3A antibody (M00252) produced clear, specific bands with low background, and reliably detected expected changes in FOXO3A levels in normal, Alzheimer’s model, and treated mouse hippocampal tissues, demonstrating excellent performance.

Excellent, submitted by Huili Yin, Shandong First Medical University on 2026-03-30

SKU	M00252
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	① Normal mouse hippocampal tissue, ② Hippocampal tissue from Alzheimer’s disease model mouse, ③ Hippocampal tissue from Alzheimer’s disease model mouse treated with a self-developed drug. Total protein was extracted from all samples.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	TNF alpha Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	FOXO3a is a key member of the FOXO transcription factor family, playing a central regulatory role in cellular processes such as stress response, apoptosis, autophagy, metabolism, and antioxidation. In Alzheimer’s disease, FOXO3a is known to exert neuroprotective effects. Experimental results show that FOXO3a protein levels are significantly decreased in the brains of Alzheimer’s model mice, while treatment leads to a noticeable increase.

The PI3K p85 alpha Antibody (M00318-2) showed clear and specific bands in WB of rat skeletal muscle, with PI3K p85 expression increased in the model group and decreased in a dose-dependent manner in the treatment groups, consistent with expectations.

Excellent, submitted by Guangtian Yu, Ningxia Medical University on 2026-03-26

SKU	M00318-2
Application	Western Blot
Sample	Rat skeletal muscle tissue
Sample Processing Description	Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer,Blocking buffer
Primary Antibody	PI3 Kinase p85 alpha Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	Based on the experimental results, the band of PI3K p85 is clear. The PI3K p85 level in the model group is significantly higher than in the normal group, whereas in the treatment group, the PI3K p85 level decreases with increasing drug dose, which is consistent with expectations.

The AKT1 Antibody (M00024-1) showed clear, specific bands in WB of rat skeletal muscle, with expression changes consistent with expectations.

Excellent, submitted by Guangtian Yu, Ningxia Medical University on 2026-03-25

SKU	M00024-1
Application	Western Blot
Sample	Rat skeletal muscle tissue
Sample Processing Description	Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	AKT1 Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	The AKT1 bands are clear and correctly positioned; expression is significantly higher in the model group than in the control, and decreases with increasing drug dose in the treatment groups, consistent with expectations.

MTOR Antibody (M00003) WB on rat skeletal muscle shows clear bands, with higher MTOR in the model and dose-dependent reduction in treatment groups.

Excellent, submitted by Guangtian Yu, Ningxia Medical University on 2026-03-24

SKU	M00003
Application	Western Blot
Sample	Rat skeletal muscle tissue
Sample Processing Description	Total protein was extracted from rat skeletal muscle tissue: ① normal, ② injury model, ③ high-dose drug treatment, ④ medium-dose drug treatment, ⑤ low-dose drug treatment.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	mTOR/Tor Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The results show clear MTOR bands, with higher levels in the injury model compared to normal, and a dose-dependent decrease in the treatment groups, as expected.

This Western blot experiment using COL3A1 antibody (M00788-1) on mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses.

Excellent, submitted by Huanping Qian, Ningxia Medical University on 2026-03-24

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SKU	M00788-1
Application	Western Blot
Sample	mouse cardiac tissue
Sample Processing Description	Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	Collagen III Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h at RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	This experiment aimed to investigate changes in COL3A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses.

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The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by Huanping Qian, Ningxia Medical University on 2026-03-24

SKU	M01072-1
Application	Western Blot
Sample	mouse cardiac tissue
Sample Processing Description	Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h at RT
Detection	Substrate: ECL substrate Imaging system: ChemiDoc MP
Results Summary	This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

Western blot analysis using Anti-COL3A1 Antibody (M00788-1) showed clear and specific bands in mouse myocardium samples from 3-, 6-, 12-, and 18-month-old mice, with overall comparable COL3A1 expression and a slight, non-significant increase with age.

Excellent, submitted by Huanping Qian, Ningxia Medical University on 2026-03-05

SKU	M00788-1
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Myocardial tissues were collected from mice at 3, 6, 12, and 18 months of age, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, blocking buffer
Primary Antibody	Collagen III Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	This experiment aimed to study the changes in COL3A1 protein in mouse myocardium at 3, 6, 12, and 18 months of age (representing young, adult, middle-aged, and old mice). The results show that COL3A1 levels are largely similar across ages, with a slight, non-significant increase as age progresses.