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In WB using β-tubulin antibody (Cat# A01857-1), a clear, specific band was observed in OCI-LY8 cells with clean background, showing stable expression after SRPK1 knockdown.

Excellent, submitted by on
SKU A01857-1
Application Western Blot
Sample human OCI-LY1 cells
Sample Processing Description Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio and boiled for 10 minutes. Fifteen microliters of each sample were loaded per well.
Other Reagents 5% Non-fat milk
Primary Antibody Beta Tubulin/TUBB Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary The antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has clear and well-defined edges.

The SLC40A1 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were observed, the differences in expression levels were still clearly discernible.

Excellent, submitted by on
SKU A01953-2
Application Western Blot
Sample human uterine tissue
Sample Processing Description The tissue was minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody Anti-SLC40A1 Antibody Picoband®
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary The SLC40A1 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were detected, the differences in expression levels were still clearly observable and did not affect the analysis of the experimental results.

The SLC7A11 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were observed in the experiment, this did not affect the interpretation of the results.

Excellent, submitted by on
SKU A03036-2
Application Western Blot
Sample human uterine tissue
Sample Processing Description The tissue was minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel.
Other Reagents 5% Non-fat milk
Primary Antibody Anti-xCT/SLC7A11 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary The SLC7A11 antibody was used to detect the expression of the target protein in human uterine tissue. Although two bands were observed in the experiment, this did not affect the interpretation of the results.

This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.

Excellent, submitted by on
SKU M00006-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody Anti-PTEN Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.

The antibody shows clear bands with no non-specific signals in WB, and is suitable for detecting PIK3R1 protein in mouse hippocampus by Western blot.

Excellent, submitted by on
SKU A00318-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA lysis buffer containing protease inhibitors, followed by protein quantification. The samples were then mixed with 5× protein loading buffer and heated for 10 minutes for denaturation. Five microliters of protein sample were loaded into each lane for SDS-PAGE.
Primary Antibody Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent , Imaging system:ChemiDoc MP
Results Summary The antibody shows clear bands with no non-specific signals in WB, and is suitable for detecting PIK3R1 protein in mouse hippocampus by Western blot.

The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The WB results showed clear bands, and the antibody could be reused after recovery with good performance, offering excellent cost-effectiveness.

Excellent, submitted by on
SKU M02059
Application Western Blot
Sample Mouse Uterus tissue
Sample Processing Description The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The Western blot results showed clear bands, and the antibody retained good performance after reuse, offering excellent cost-effectiveness.
Primary Antibody Anti-GPX4 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
The GPX4 antibody was used to detect the protein expression in mouse uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse, providing excellent cost-effectiveness.

Western blot analysis was performed using the GP9 antibody to detect GP9 protein expression in the mouse hippocampus. This antibody is highly efficient and specific, making it suitable for quantitative WB detection of GP9 in mouse tissues.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Primary Antibody Anti-AKT1 Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear and free of non-specific signals. After recovery, the antibody could be reused and still showed good performance.

The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance.

Excellent, submitted by on
SKU M02401
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The tissue was minced and sonicated, then lysed on ice for 1 hour using RIPA buffer. After centrifugation to collect the supernatant and protein quantification by BCA, samples were mixed with loading buffer at the appropriate ratio and denatured by boiling in a water bath. Fifteen microliters of each protein sample were loaded per lane onto SDS-PAGE gel.
Primary Antibody Anti-Ferritin FTH1 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance.

Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus.

Excellent, submitted by on
SKU M02226
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed in RIPA buffer supplemented with a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE.
Primary Antibody Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:ChemiDoc MP
Results Summary Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus. Although minor non-specific bands were observed, they did not affect the trend analysis, indicating that the antibody is suitable for detecting the target protein in this tissue.

The antibody was used to perform WB quantitative detection of ALDH2 protein in mouse hippocampus. The experimental results showed clear and distinct bands, indicating that it is suitable for WB detection of ALDH2 protein in mouse tissues.

Excellent, submitted by on
SKU PB9472
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus tissue was lysed using RIPA lysis buffer supplemented with a protease inhibitor cocktail. Protein concentration was determined, and samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE.
Primary Antibody Anti-ALDH2 Antibody Picoband®
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system: ChemiDoc MP (Bio-Rad)
Results Summary The antibody was used for quantitative WB detection of ALDH2 protein in mouse hippocampus. The results showed clear and distinct bands, indicating its suitability for WB detection of ALDH2 protein in mouse tissues.