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PB9920 successfully extracted rat osteocalcin from rat EDTA plasma

--Chad Borges, Arizona State University, Biodesign Virginia G. Piper Center for Personalized Diagnostics, Assistant Professor

Excellent, submitted by on

Source: Customer Feedback Submission

SKU PB9920
Applications Picomole scale immunoprecipitation
Sample Rat EDTA plasma
Detection Bruker maXis 4G mass spectrometer (LC-MS; Q-TOF)

"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."

Boster's Anti-MDM2 Antibody Worked!

--Stacy Wilson, Biology, REVOLUTION Medicines, Inc., SRA

Excellent, submitted by on

Source: Biocompare.com

SKU PA1378-1
Applications Western Blot
Sample MDA-MB-468 and HEK293E cell lysates
Detection SuperSignal West Pico Plus with a BioRad imager

"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."

The signal was very strong and clean!

--Chi Li, MEDICINE, UNIVERSITY OF LOUISVILLE, PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

SKU PB9212
Applications Western Blot
Sample Whole cell extract of pancreatic tumor cell MIA PaACa2
Detection Enhanced chemiluminescence

"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."

BosterBio Sparcl1 Works

--Ruby C, NEUROSCIENCE, TUFTS UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PA2088
Applications Immunohistochemistry
Sample Mouse astrocytes
Detection Confocal/Fluorescent Scope

"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."

Good enough for publishing

--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9333
Applications Western Blot
Sample Human breast cancer cell line
Detection Bio-Rad imager

"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."

We got bands as expected...

--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS

Excellent, submitted by on

Source: Biocompare.com

SKU PA1129-2
Applications Western Blot
Sample Mouse lung lysates
Detection SuperSignal West Femto Maximum Sensitivity Substrate

"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."

Specific and low variability between lots

--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST

Excellent, submitted by on

Source: Biocompare.com

SKU RP1021
Applications ELISA
Sample Purified mouse prolactin and plasma
Detection TMB Substrate

"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."

Anti GRK5 (PB 9708) is useful, good.

--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9708
Applications Western Blot
Sample Rat endothelial cells
Detection Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."

Reliable and robust

--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN

Excellent, submitted by on
Rat il 6 elisa kit

Source: Biocompare.com

SKU PA1340
Applications Western Blot
Sample Cell lysate
Detection Enhanced chemiluminescence

"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

Working Antibody for Versican

--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER

Excellent, submitted by on

Source: Biocompare.com

SKU PB9453
Applications Western Blot
Sample Human cell lines
Detection ECL

"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."