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This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.

Excellent, submitted by on
SKU M00006-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Other Reagents 5% Non-fat milk
Primary Antibody Anti-PTEN Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent, Imaging system:ChemiDoc MP
Results Summary This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.

The antibody shows clear bands with no non-specific signals in WB, and is suitable for detecting PIK3R1 protein in mouse hippocampus by Western blot.

Excellent, submitted by on
SKU A00318-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA lysis buffer containing protease inhibitors, followed by protein quantification. The samples were then mixed with 5× protein loading buffer and heated for 10 minutes for denaturation. Five microliters of protein sample were loaded into each lane for SDS-PAGE.
Primary Antibody Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent , Imaging system:ChemiDoc MP
Results Summary The antibody shows clear bands with no non-specific signals in WB, and is suitable for detecting PIK3R1 protein in mouse hippocampus by Western blot.

The HIF1A antibody was used to detect the expression of the target protein in human uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Primary Antibody Anti-HIF-1 alpha Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary The HIF1A antibody was used to detect the expression of the target protein in human uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse. It offers excellent cost-effectiveness, with a clear advantage over other antibodies with similar specifications. Highly recommended for use!

The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The WB results showed clear bands, and the antibody could be reused after recovery with good performance, offering excellent cost-effectiveness.

Excellent, submitted by on
SKU M02059
Application Western Blot
Sample Mouse Uterus tissue
Sample Processing Description The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The Western blot results showed clear bands, and the antibody retained good performance after reuse, offering excellent cost-effectiveness.
Primary Antibody Anti-GPX4 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
The GPX4 antibody was used to detect the protein expression in mouse uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse, providing excellent cost-effectiveness.

The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance.

Excellent, submitted by on
SKU M02401
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The tissue was minced and sonicated, then lysed on ice for 1 hour using RIPA buffer. After centrifugation to collect the supernatant and protein quantification by BCA, samples were mixed with loading buffer at the appropriate ratio and denatured by boiling in a water bath. Fifteen microliters of each protein sample were loaded per lane onto SDS-PAGE gel.
Primary Antibody Anti-Ferritin FTH1 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon
Results Summary The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance.

Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus.

Excellent, submitted by on
SKU M02226
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed in RIPA buffer supplemented with a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE.
Primary Antibody Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:ChemiDoc MP
Results Summary Western blot analysis was performed using the COL6A1 antibody to detect COL6A1 protein expression in the mouse hippocampus. Although minor non-specific bands were observed, they did not affect the trend analysis, indicating that the antibody is suitable for detecting the target protein in this tissue.

The antibody was used to perform WB quantitative detection of ALDH2 protein in mouse hippocampus. The experimental results showed clear and distinct bands, indicating that it is suitable for WB detection of ALDH2 protein in mouse tissues.

Excellent, submitted by on
SKU PB9472
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus tissue was lysed using RIPA lysis buffer supplemented with a protease inhibitor cocktail. Protein concentration was determined, and samples were mixed with 5× protein loading buffer and denatured by heating at 100°C for 10 minutes. Five microliters of each protein sample were loaded per lane onto SDS-PAGE.
Primary Antibody Anti-ALDH2 Antibody Picoband®
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system: ChemiDoc MP (Bio-Rad)
Results Summary The antibody was used for quantitative WB detection of ALDH2 protein in mouse hippocampus. The results showed clear and distinct bands, indicating its suitability for WB detection of ALDH2 protein in mouse tissues.

Western blot analysis of AKT1 protein in mouse hippocampus using the AKT1 antibody showed clear bands. The antibody is suitable for mouse hippocampal tissue samples.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Primary Antibody Anti-AKT1 Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system: ChemiDoc MP (Bio-Rad)
Results Summary Western blot analysis of AKT1 protein in the mouse hippocampus using the AKT1 antibody showed clear bands. This antibody is suitable for mouse hippocampal tissue samples.

A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Immunofluorescence
Sample Human HaCaT cell
Sample Processing Description Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody.
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:100-1:200, overnight at 4 ℃
Secondary Antibody Goat anti-rabbit IgG conjugated to Alexa Fluor 488
Secondary Incubation 1:1000, 1-2 hours in room temperature
Other Reagents used PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining
Detection Fluorescence microscopy using AlexaFluor488 (excitation 488 nm, emission 519 nm) using Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary The CyclinE-N15 antibody (DZ41310) worked fine in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for D15 transcript Cyclin E detection in human cell systems.

Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Western Blot
Sample Human HaCaT cell, Human A2780 cell, Human HCT cell
Sample Processing Description Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation 1:10000, 1-2 hours in room temperature
Detection Biorad Chemidoc
Results Summary Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.