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| SKU | DZ4103 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution. |
| SKU | DZ41032 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution. |
Source: Biocompare.com
| SKU | PA1021-2 |
|---|---|
| Application | Western Blot |
| Sample | Tumor cell lysate |
| Primary Incubation | Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw |
| Blocking Agent | 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) |
| Secondary Incubation | Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw |
| Tertiary Incubation | HRP-bound secondary antibodies were detected by WestPico from ThermoScientific/Tw |
| Detection | Chemiluminescence: West Pico from Thermo Scientific |
| Results Summary | The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20℃. |
"The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane."
Source: Biocompare.com
| SKU | PA1667 |
|---|---|
| Application | Western Blot |
| Sample | 22RV1 cytosolic and membrane fractions |
| Primary Incubation | 1:1000, overnight at 4 degree |
| Blocking Agent | 5% Milk |
| Secondary Incubation | 1:5000, 1 hour RT. |
| Detection | ECL |
| Results Summary | Please refer our paper (Scientific Reports 6, Article number: 23135 (2016)doi:10.1038/srep23135). |
"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."
Source: Biocompare.com
| SKU | P01424-1 |
|---|---|
| Application | Flow Cytometry |
| Sample | B cells |
| Primary Incubation | 0.25 µl/10^6 cells 50min RT |
| Blocking Agent | FBS 5% |
| Secondary Incubation | F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6 |
| Detection | Flow Cytometry |
| Results Summary | Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody. |
Source: Biocompare.com
| SKU | DZ41142 |
|---|---|
| Application | Western Blot |
| Sample | Human cell lines |
| Primary Incubation | 1:500 with 1% BSA in TBS, incubated overnight, at 4oC, with shaking |
| Blocking Agent | 5% BSA in TBS |
| Secondary Incubation | Licor IRDye 800 Goat anti Rabbit diluted 1:10000 with 1% BSA in TBS, incubated for 1 hour at RT , with shaking |
| Detection | Licor Odyssey IR Scanner |
| Additional Notes | The antibody has very good stability at room temperature (transit took over 2 weeks and the ice packs had long melted before delivery). |
| Results Summary | The antibody gave a detectable band at the expected size for all cell lines tested with no non-specific bands. |
"This antibody was used to determine the level of protein expression in various cell lines in order to set up two groups (high vs low expression). This antibody was custom made because numerous suppliers have either stopped their production for this antibody or quality/specificity was low."
Source: Biocompare.com
| SKU | A00633-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Neutrophils |
| Primary Incubation | 1:2000 1 hr |
| Blocking Agent | 5% BSA |
| Secondary Incubation | 1:3000 |
| Detection | HRP |
| Results Summary | Nicely compared total LTF in neutrophils to degranulated LTF. In the image below, Lane 1: Total LTF from mouse neutrophil cell lysates. Lane 2: degranulated LTF. Ratio 1:20. |
"Nicely detects degranulated LTF in supernatant mouse neutrophils stimulated with bacteria. Used at 1:2000 in 5% BSA."
Source: Biocompare.com
| SKU | M06465 |
|---|---|
| Application | Western Blot |
| Sample | Hello cell and mouse tissues |
| Primary Incubation | 1:2000 dilution at 4oC overnight |
| Blocking Agent | 5% non-fat milk |
| Secondary Incubation | 1:2500 dilution at RT for 1 hour |
| Detection | Fluorescence |
| Results Summary | This antibody works in WB with tested samples. The signal is strong enough to get a decent image. It works for human and mouse samples. |
"This antibody of MTHFD2 is good for WB with mouse tissue and human cell. Signal is strong and the background is ok."
Source: Biocompare.com
| SKU | A05219 |
|---|---|
| Application | Western Blot |
| Sample | HEK293T |
| Primary Incubation | 1:5000 for 24hours at 4degrees celsius. |
| Blocking Agent | Skim milk |
| Secondary Incubation | 1 hour room temperature 1:3000 |
| Detection | ECL |
| Results Summary | We silenced ULK1 and ULK2 genes in HEK293T cells, the antibody could differentiate the two proteins. |
"Used for western blotting. The antibody worked good, used skim milk for blocking. It was used for investigating silenced HEK293T cells."
Source: Biocompare.com
| SKU | DZ41383 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish WKM, IPEX and FFPE tissue sections |
| Primary Incubation | Overnight |
| Blocking Agent | 3% goat serum in PBS |
| Secondary Incubation | 1 hour |
| Tertiary Incubation | None |
| Detection | Fluorescence microscopy |
| DOI or PMID # | https://doi.org/10.1101/2024.04.29.591640 |
| Results Summary | Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF. |
"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."