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WB of COL1A1 (Cat# PA2140-1) in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL1A1 levels clearly increase with age.

Excellent, submitted by Huanping Qian, Ningxia Medical University on 2026-03-24

SKU	PA2140-1
Application	Western Blot
Sample	mouse cardiac tissue
Sample Processing Description	Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	Collagen I/COL1A1 Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system: ChemiDoc MP
Results Summary	This experiment aimed to investigate changes in COL1A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL1A1 levels clearly increase with age.

The Anti-α-SMA antibody (Cat# BM3902) shows clear and specific bands with low background in WB analysis of mouse heart tissues across different ages, with results consistent with expected trends.

Excellent, submitted by Huanping Qian, Ningxia Medical University on 2026-03-24

SKU	M01072-1
Application	Western Blot
Sample	mouse cardiac tissue
Sample Processing Description	Mouse cardiac tissues were collected from 3-, 6-, 12-, and 18-month-old mice, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	alpha smooth muscle Actin ACTA2 Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h at RT
Detection	Substrate: ECL substrate Imaging system: ChemiDoc MP
Results Summary	This experiment aimed to investigate the changes in α-SMA expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, and aged stages). The results show that α-SMA levels are generally similar across different ages, with a slight but not significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by Yetao Ju, Liaoning University of Traditional Chinese Medicine on 2026-03-05

SKU	M00057-2
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	goat anti rabbit secondary antibodies
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by Yetao Ju, Liaoning University of Traditional Chinese Medicine on 2026-02-28

SKU	PA2087
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents	blocking buffer
Primary Antibody	Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:2000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

Using Boster’s IL-6 antibody (RP1012) in WB on HK-2 cells produced clear, specific bands with high sensitivity and cost-effectiveness, outperforming previously tested antibodies.

Excellent, submitted by Jingwen Zhang, Ocean University of China on 2026-02-27

SKU	RP1012
Application	Western Blot
Sample	human HK-2 cells
Sample Processing Description	Cell samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured at 98 °C. Twenty microliters of each protein sample were loaded per lane onto SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Interleukin-6 IL6 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate
Results Summary	Previously, WB experiments were performed using antibodies from two domestic and international suppliers, which showed poor specificity and were expensive. Subsequently, Boster’s IL-6 antibody (Cat. RP1012) was used, demonstrating high specificity, strong titer, cost-effectiveness, and yielding clear bands.

Pancreatic IL-6 levels measured using the Boster Anti-IL-6 antibody (PB9034) demonstrated high specificity and sensitivity in WB analysis, providing reliable evidence for evaluating the anti-inflammatory effect of the nanodrug.

Excellent, submitted by Jiahui Yan, College of Chemistry and Chemical Engineering, Ocean University of China on 2026-02-27

SKU	PB9034
Application	Western Blot
Sample	mouse PACs cells
Sample Processing Description	After centrifugation, the supernatant was collected. A small aliquot of the protein solution was taken for BCA quantification, and the remaining protein solution was mixed with an equal volume of loading buffer, then denatured in a 100 °C water bath for 5 minutes.
Other Reagents	Protein-Free Rapid Blocking Buffer
Primary Antibody	Anti-IL6 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	The levels of IL-6 and IL-1β in the pancreas are key indicators for evaluating whether the nanodrug exerts anti-inflammatory effects. Initially, antibodies from two domestic and international suppliers were used for WB experiments, but their specificity was relatively poor. Subsequently, the antibody from Boster was adopted, which demonstrated strong specificity, high titer, and cost-effectiveness.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-02-26

SKU	PB9533
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents	5% Non-fat milk
Primary Antibody	CDK1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In IHC using Ki67 antibody (Cat# PB9026), strong and specific nuclear staining was observed in nude mouse tumor tissue, confirming high Ki67 expression and active cell proliferation.

Excellent, submitted by Jin Sun, Tangdu Hospital, Xi’an, China on 2026-02-26

SKU	PB9026
Application	Western Blot
Sample	Nude mouse tumor tissue
Sample Processing Description	Paraffin-embedded tumor tissue sections.
Primary Antibody	Ki67/MKI67 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary	This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

In this IHC experiment using Anti-CD4 antibody (Cat# M00344) on human cervical cancer paraffin sections, CD4-positive helper T cells were clearly and specifically stained with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by Yanan Peng, The First Affiliated Hospital of Shihezi University on 2026-02-25

SKU	M00344
Application	Western blot
Sample	human cervical cancer paraffin sections
Sample Processing Description	Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other Reagents	Goat serum, DAB substrate solution
Primary Antibody	CD4 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Two-step IHC detection kit
Secondary Incubation	30 min at 37℃
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	CD4 is a marker of helper T cells; in this IHC experiment, CD4 staining clearly identified helper T cells in human cervical cancer samples, showing excellent specificity and performance of the antibody.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	M00865
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	AQP1 Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.