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Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by on
SKU M00057-2
Application Western Blot
Sample mouse brain tissue
Sample Processing Description Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents 5% non-fat milk
Primary Antibody GAPDH Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody goat anti rabbit secondary antibodies
Secondary Incubation 1:5000, 1 h in RT
Detection Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.

Excellent, submitted by on
SKU M01394-4
Application Immunofluorescence
Sample mouse brain tissue
Sample Processing Description Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded.
Other ReagentsGoat serum, DAPI Staining Solution, Antifade fluorescence mounting medium.
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DMi3000
Results Summary IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology.

In this IHC experiment using Anti-SLC22A3 antibody (Cat# M04914), strong and clear expression was observed in cervical cancer tumor cells, with further comparison planned between tumor and adjacent tissues.

Excellent, submitted by on
SKU M04067-2
Application Immunohistochemistry
Sample human cervical cancer tissue
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody SLC22A3 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary IHC using SLC22A3 antibody (M04914) showed strong and clear expression in tumor cells of human cervical carcinoma, and the next step will be to compare expression between tumor and adjacent normal tissue.

In this IHC experiment using Anti-SERPINB3 antibody (Cat# M04067-2) on human cervical cancer paraffin sections, SERPINB3 was highly expressed in tumor cells with clear positive staining and low background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M04067-2
Application Immunohistochemistry
Sample human cervical cancer tissue
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody SerpinB3 Rabbit Monoclonal Antibody
Primary Incubation 1:400, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary The results demonstrated high expression of SERPINB3 in cervical cancer tumor cells with clear positive staining; further studies will compare its expression levels between tumor and adjacent non-tumor tissues.

In this IHC experiment using Anti-CD4 antibody (Cat# M00344) on human cervical cancer paraffin sections, CD4-positive helper T cells were clearly and specifically stained with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by on
SKU M00344
Application Western blot
Sample human cervical cancer paraffin sections
Sample Processing Description Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other ReagentsGoat serum, DAB substrate solution
Primary Antibody CD4 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC detection kit
Secondary Incubation 30 min at 37℃
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary CD4 is a marker of helper T cells; in this IHC experiment, CD4 staining clearly identified helper T cells in human cervical cancer samples, showing excellent specificity and performance of the antibody.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by on
SKU M00865
Application Western blot
Sample MADB106 rat mammary carcinoma cells
Sample Processing Description MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other ReagentsRIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody AQP1 Rabbit Monoclonal Antibody
Primary Incubation 1:3000, overnight at 4 ℃
Secondary Antibody 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation 1h at RT
Detection Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by on
SKU M00297
Application Immunocytochemistry
Sample mouse brain
Sample Processing Description Normal brain tissue and brain tissue from a cerebral infarction model
Other ReagentsGoat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by on
SKU M00422
Application Immunohistochemistry
Sample human liver cancer
Sample Processing Description Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other ReagentsGoat serum, DAB Chromogenic Solution
Primary Antibody FAP1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by on
SKU M01917
Application Immunofluorescence
Sample mouse brain
Sample Processing Description Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other ReagentsGoat serum，DAPI，Anti-fade mounting medium
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody 1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

Using IHC on paraffin-embedded human placental tissues, the Anti-Histone H3 (acetyl K27) antibody (M12477-15) showed high specificity and clear staining, with higher H3K27 acetylation in preterm placentas than in normal controls.

Excellent, submitted by on
SKU M12477-15
Application Immunohistochemistry
Sample normal and preterm human placentas
Sample Processing Description Clinically collected normal and preterm placentas were sectioned longitudinally (with amnion and chorion visible) and prepared as paraffin-embedded sections.
Other ReagentsTris-EDTA Antigen Retrieval Solution, DAB Chromogenic Solution
Primary Antibody Histone H3 (acetyl K27) Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Polymer anti-rabbit IgG-HRP IHC detection kit
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Histone H3 (acetyl K27) is the acetylated form of histone H3 at lysine 27 and serves as a sensitive indicator of epigenetic abnormalities in placental cells; IHC results showed higher expression in preterm placentas than in normal placentas.