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The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by Fei Long, Zhongnan Hospital of Wuhan University on 2026-02-12

SKU	M00422
Application	Immunohistochemistry
Sample	human liver cancer
Sample Processing Description	Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other Reagents	Goat serum, DAB Chromogenic Solution
Primary Antibody	FAP1 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation	30 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by Zengting Li, Huazhong University of Science and Technology on 2026-02-12

SKU	M01917
Application	Immunofluorescence
Sample	mouse brain
Sample Processing Description	Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other Reagents	Goat serum，DAPI，Anti-fade mounting medium
Primary Antibody	Iba1 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

This antibody is highly specific and efficient, suitable for detecting STAT3 protein in rat colon by Western blot, with clear results.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-08

SKU	M00007
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows Western blot results of the target protein STAT3 and the loading control Actin in the colon of normal rats, model rats, low/medium/high dose Chinese medicine treatment groups, and Western medicine treatment group. No differences were observed between the groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of STAT3 (Phospho-Y705) protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-07

SKU	P00007-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of the target protein STAT3 (Phospho-Y705) and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting RHOA/B/C protein in rat colon by Western blot, with only minimal non-specific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00207-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Rho A + B + C Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein RHOA/B/C and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of PTEN protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00006-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	PTEN Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows a schematic of Western blot results for the target protein PTEN and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

This antibody exhibits high efficiency and specificity and is suitable for Western blot detection of CCND1 protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00149-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Cyclin D1 CCND1 Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein CCND1 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting Bcl-XL protein in rat colon by Western blot, with only minimal nonspecific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2025-12-30

SKU	M00181-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Bcl-XL BCL2L1 Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows a schematic representation of Western blot results for the target protein Bcl-XL and the internal control Actin in rat colon across the normal rat group, model group, traditional Chinese medicine group, and western medicine group. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of AKT1 (Phospho-T450) protein in rat colon, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2025-12-26

SKU	P00024-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer supplemented with PMSF (100:1) was used to lyse the samples for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected. Fivefold loading buffer was added, and the samples were denatured at 100 °C for 10 min before loading onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:2000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of AKT1 (Phospho-T450) and the internal control Actin in rat colon tissues from different groups. Among the low, medium, and high doses of the traditional Chinese medicine, the high-dose group exhibits the best therapeutic effect. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of STAT3 protein in MCF-7 cells, with only minor nonspecific bands observed.

Excellent, submitted by Siyuan Bu, LUTCM on 2025-12-26

SKU	M00007
Application	Western Blot
Sample	Human mcf-7 cells
Sample Processing Description	RIPA lysis buffer supplemented with a protease inhibitor cocktail was added to the cell culture dish and incubated on ice for 30 min. The lysates were then centrifuged, and the supernatants were collected. Protein concentration was determined, and samples were adjusted accordingly. Five times protein loading buffer was added, and the samples were denatured at 100 °C for 10 min. A total of 15 μL of protein sample was loaded per lane for SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	STAT3 Rabbit Monoclonal Antibody
Primary Incubation	1:500, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:2000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	This antibody is highly specific and efficient, suitable for Western blot detection of STAT3 protein in MCF-7 cells, with only minor nonspecific bands observed.