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Source: Biocompare.com
| SKU | A00625 |
|---|---|
| Application | Western Blot |
| Sample | Human Tissue |
| Detection | Fluorescent based |
"We are comparing the expression of the TRPC6 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC6 antibody worked perfectly in detection in an Alzheimer's tissue sample. No extra bands. I recommend."
Source: Biocompare.com
| SKU | PB9271 |
|---|---|
| Application | Western Blot |
| Sample | Human Tissue |
| Detection | Fluorescent based |
"We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 antibody worked perfectly in detection in human tissue. Perfect band obtained at desired molecular weight. No extra bands. I recommend."
Source: Biocompare.com
| SKU | PA1385 |
|---|---|
| Application | Western Blot |
| Sample | Human NSC cell lysates |
| Detection | ECL chemiluminescence |
"We study neurodegeneration in our lab and I was looking for an antibody for human TIMP-1. The antibody detects a band at 28kD, which is the predicted molecular weight of TIMP-1. Good antibody for TIMP-1, cleaner than other antibodies I have tested. In summary, this antibody is good for human samples and I highly recommend it."
| SKU | PB9094 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Human Bowel |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 min |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | at 37°C for 30 min |
Source: Biocompare.com
| SKU | PB10032 |
|---|---|
| Applications | Western Blot |
| Sample | Human liver, rat liver, mouse liver, mouse kidney, and mouse brain |
| Detection | Typhoon 940 |
"We are studying the metabolism of amino acid and we are interested in knowing if the pathway involved this protein has changed under the background of a knockout mouse. The western blot image for this antibody looks clean and the signal looks strong. The band shown on western blot is at the right size and the intensity is strong enough. Equally important, the price is generally lower than other companies. This antibody works ok in western blot experiments with human, rat, and mouse tissues."
Source: Customer Feedback Submission
| SKU | PA1373 |
|---|---|
| Applications | Western Blot |
| Sample | Mouse brain lysate |
| Detection | Licor Imaging and ImageJ |
"We have tested PA1373 trial sample in our lab and it works far better compared to other vendors. We are extremely happy to get the results. We looked for 5HT2A expression in various brain regions. Our bands show clear expression level of 5HT2A. We now plan to buy this antibody for all our laboratory needs."
Source: Customer Feedback Submission
| SKU | PB9920 |
|---|---|
| Applications | Picomole scale immunoprecipitation |
| Sample | Rat EDTA plasma |
| Detection | Bruker maXis 4G mass spectrometer (LC-MS; Q-TOF) |
"The immobilized antibody successfully extracted rat osteocalcin from rat EDTA plasma. The results shown align very well with mass and known posttranslational modifications of rat osteocalcin."
Source: Biocompare.com
| SKU | PA1378-1 |
|---|---|
| Applications | Western Blot |
| Sample | MDA-MB-468 and HEK293E cell lysates |
| Detection | SuperSignal West Pico Plus with a BioRad imager |
"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."
Source: Biocompare.com
| SKU | PB9212 |
|---|---|
| Applications | Western Blot |
| Sample | Whole cell extract of pancreatic tumor cell MIA PaACa2 |
| Detection | Enhanced chemiluminescence |
"Thirty micrograms of whole cell extract of pancreatic tumor cell MIA PaCa-2 were loaded on a 4-12% Bis-Tris gel. The membrane was blotted with 1xPBS + 0.2% Tween-20 + 10% non-fat dry milk. The membrane was incubated with Anti-HMOX1 Antibody (PB9212) at the concentration of 200 ng/ml at room temperature for 3 hours. The membrane was then blotted with HRP-conjugated anti-rabbit secondary antibodies at 4oC overnight. The band was detected using regular ECL with 1 minute exposure. A major band with 32 kD was detected."
Source: Biocompare.com
| SKU | PA2088 |
|---|---|
| Applications | Immunohistochemistry |
| Sample | Mouse astrocytes |
| Detection | Confocal/Fluorescent Scope |
"The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data. The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size. The bottom line: Good."