Customer Testimonials

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Good enough for publishing

--Chun-Chih Tseng, NORRIS CANCER CENTER, UNIVERSITY OF SOUTHERN CALIFORNIA, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9333
Applications Western Blot
Sample Human breast cancer cell line
Detection Bio-Rad imager

"I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44. CD44 has multiple spliced isoforms. The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing. Relatively inexpnsive, okay specificity. Good enough to detect CD44 C-term."

We got bands as expected...

--Christina Klinger, DEPT. OF TRANSLATIONAL AND REGENERATIVE MEDICINE, UNIVERSITY OF ARIZONA, MANAGER OF LABORATORY OPERATIONS

Excellent, submitted by on

Source: Biocompare.com

SKU PA1129-2
Applications Western Blot
Sample Mouse lung lysates
Detection SuperSignal West Femto Maximum Sensitivity Substrate

"We sought to see differences in expression of EPAS1 in protein from mouse lung samples. We selected this product because we have used other antibodies from Boster in the past with success. We got bands as expected, but they were not indicative of good conjugation as they were very light. We believe this is because we did not use the recommended Enhanced Chemiluminscent kit with anti-rabbit IgG (EK1002), so we will repeat the experiment with this chemi kit before concluding whether the antibody worked or not. This antibody should work using the chemiluminescent kit recommended...Heed the recommendation of the website and use the chemi kit suggested for high quality visualization."

Specific and low variability between lots

--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST

Excellent, submitted by on

Source: Biocompare.com

SKU RP1021
Applications ELISA
Sample Purified mouse prolactin and plasma
Detection TMB Substrate

"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."

Anti GRK5 (PB 9708) is useful, good.

--Yongzhen Guo, EMERGENCY MEDICINE, THOMAS JEFFERSON UNIVERSITY, RESEARCH ASSISTANT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9708
Applications Western Blot
Sample Rat endothelial cells
Detection Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

"After using this antibody for western blot to analyze rat endothelial cells' protein, I found PB9708 Anti-GRK5 antibody worked very well. The blot of 68 Kda can be seen, however, we can see another 3 blots at least. In a word, Anti GRK5 (PB 9708) is useful and good. The blot of 68 Kda can be detected."

Reliable and robust

--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN

Excellent, submitted by on
Rat il 6 elisa kit

Source: Biocompare.com

SKU PA1340
Applications Western Blot
Sample Cell lysate
Detection Enhanced chemiluminescence

"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."

Working Antibody for Versican

--Gang Ye, BIOLOGY, YORK UNIVERSITY, RESEARCH ASSOCIATE/LAB MANAGER

Excellent, submitted by on

Source: Biocompare.com

SKU PB9453
Applications Western Blot
Sample Human cell lines
Detection ECL

"Since versican has several isoforms, the current antibodies targeting versican on the market is very tricky. Most of them didn't give us a reasonable result. Some of them only detect a band at 70 KD, which is unlikely a potent component of versican protein. Bosterbio claims this antibody dectects a band over 250 KD. Since versican is a very big protein. It sounds this is a real antibody. After testing, we found we can see a clear detected band around 245 KD. We realized that this might be the true result...Although 245 KD is still smaller than the versican core protein, it is likely the true result of versican dectection."

High specificity and stability

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

SKU PB9333
Applications Western Blot
Sample Tumor cell lysate and recombinant protein
Detection SuperSignal West Pico Chemiluminescent Substrate

"The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting."

I would highly recommend this Boster antibody...

--Elena Deryugina, CELL AND MOLECULAR BIOLOGY, THE SCRIPPS RESEARCH INSTITUTE, ASSISTANT PROFESSOR

Excellent, submitted by on

Source: Biocompare.com

SKU PB9016
Applications Western Blot
Sample Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane
Detection SuperSignal West Pico Chemiluminescent Substrate

"Rabbit antibody PB9016 from Boster was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this Boster antibody for detection of EGFR with the intact N-terminal domain."

This Antibody Works Perfectly!

--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC

Excellent, submitted by on

Source: Biocompare.com

SKU PA1239
Applications Immunohistochemistry
Sample Spinal cord
Detection Confocal microscope

"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."

Good Mouse Rad51 Antibody

--Michael Conlin, GENETICS AND MOLECULAR BIOLOGY, UNC CHAPEL HILL, GRADUATE STUDENT

Excellent, submitted by on

Source: Biocompare.com

SKU PB9323
Applications Western Blot
Sample MEFs
Detection LICOR Odyssey

"The goal was to confirm the knockdown of mouse Rad51 via siRNA. Confirmed siRNA knockdown expression. Good detection. Recommended."