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The Anti-c-FOS antibody (M00297) showed clear and specific immunofluorescence staining in mouse brain, with markedly increased c-Fos expression in neurons of the acute ischemic region compared to normal brain.

Excellent, submitted by on
SKU M00297
Application Immunocytochemistry
Sample mouse brain
Sample Processing Description Normal brain tissue and brain tissue from a cerebral infarction model
Other ReagentsGoat serum, DAB Chromogenic Solution, Anti-fade mounting medium
Primary Antibody c-Fos Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 594 Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary c-Fos is a marker of activated neural cells, especially neurons. During the acute phase of cerebral infarction, as a strong stress and injury stimulus, it drives c-Fos expression in the brain—particularly in the peri-infarct region—far higher than in normal resting brain. The experimental results show clear staining of positive cells, and the expression levels are consistent with expectations.

The Anti-FAP antibody (M00422) showed clear and specific IHC staining in human hepatocellular carcinoma, with low expression in adjacent normal tissue and strong expression in tumor stroma.

Excellent, submitted by on
SKU M00422
Application Immunohistochemistry
Sample human liver cancer
Sample Processing Description Collected clinical surgical human hepatocellular carcinoma and adjacent peritumoral tissues were fixed in formalin and embedded in paraffin.
Other ReagentsGoat serum, DAB Chromogenic Solution
Primary Antibody FAP1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Two-step IHC kit (Immunohistochemistry kit
Secondary Incubation 30 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary FAP (fibroblast activation protein) expression is mainly associated with stromal cells in the tumor microenvironment and is very low in normal human tissues. Its role in hepatocellular carcinoma (HCC) is complex, but overall it functions as an important pro-tumor factor. High FAP expression is associated with poor prognosis. It can remodel the extracellular matrix, promote tumor invasion and metastasis, and suppress anti-tumor immunity. In this study, FAP showed low expression in peritumoral tissues (close to normal tissue) and high expression in the tumor stroma of HCC.

The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by on
SKU M01917
Application Immunofluorescence
Sample mouse brain
Sample Processing Description Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other ReagentsGoat serum，DAPI，Anti-fade mounting medium
Primary Antibody Iba1 Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody 1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

In an immunofluorescence experiment using normally cultured Caco-2 cells, the ZO-1 antibody (PB9234) showed clear and specific membrane staining with low background, demonstrating reliable performance.

Excellent, submitted by on
SKU PB9234
Application Immunofluorescence
Sample human Caco-2 cell line
Sample Processing Description Cells were normally cultured in 24-well plates using MEM supplemented with 20% fetal bovine serum. When the cell density reached approximately 60%, the culture was terminated. The medium was removed, cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and then washed three times with PBS before further use.
Other ReagentsGoat serum, DAPI, and an anti-fade mounting medium.
Primary Antibody ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Caco-2 cells are a “gold standard” in vitro model for studying intestinal epithelial function, and ZO-1 is a key marker of barrier integrity. The images show immunofluorescence staining of ZO-1 in normally cultured Caco-2 cells to evaluate antibody performance. Clear membrane localization and accurate staining indicate that this antibody is suitable for downstream research applications.

Immunofluorescence using Anti-GFAP antibody (MA1045) clearly labeled protoplasmic astrocytes in the spinal cord gray matter, showing dense, bushy processes surrounding neurons, with excellent specificity and staining.

Excellent, submitted by on
SKU MA1045
Application Immunofluorescence
Sample rat spinal tissue
Sample Processing Description Paraffin-embedded transverse sections of rat spinal cord were prepared after formalin fixation.
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAPI
Primary Antibody GFAP Antibody (Monoclonal, G-A-5)
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 minutes in 37℃
Detection Imaging system:Leica DM2500
Results Summary GFAP is a marker of astrocytes. In this experiment, immunostaining for GFAP was used to label astrocytes in the gray matter of the spinal cord to observe their distribution, density, and morphology. The results showed that the labeled protoplasmic astrocytes in the gray matter had short, thick, and highly branched processes with rough surfaces, forming a dense “bushy” network tightly surrounding neuronal cell bodies and synapses, consistent with theoretical expectations and demonstrating excellent staining.

IHC using Tyrosine Hydroxylase/TH Antibody Picoband® (P00683) showed clear staining with no background, revealing markedly reduced TH expression in the medulla of Alzheimer’s mouse brain compared to normal mouse brain.

Excellent, submitted by on
SKU PB9449
Application Immunohistochemistry
Sample Normal mouse brain and Alzheimer’s model mouse brain tissue
Sample Processing Description Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain.
Other Reagents Goat serum, DAB
Primary Antibody Tyrosine Hydroxylase/TH Antibody Picoband®
Primary Incubation 1:500, overnight at 4 ℃
Secondary Antibody Two-step IHC kit
Secondary Incubation 37 minutes in 37 ℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons, specifically labeling dopaminergic, noradrenergic, and adrenergic neurons. IHC results showed that TH expression in the medulla was markedly lower in Alzheimer’s mouse compared to normal mouse.

Using Myeloperoxidase/MPO Antibody Picoband® (PA1054) in IHC, MPO showed low expression in normal mouse skin and was markedly increased in burned skin, with clear staining and expected results.

Excellent, submitted by on
SKU PA1054
Application Immunohistochemistry
Sample mouse skin tissue
Sample Processing Description Male BALB/c mice aged 6–8 weeks were used. (1) Normal dorsal skin was collected from untreated mice. (2) Burn injury was induced on the dorsal skin using a burn device. After one week of housing, hair was removed with depilatory cream. Skin samples were collected from normal mice (dorsal skin) and burned mice (burned area), fixed in formalin for 72 hours, and embedded in paraffin for sectioning./td>
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAB Chromogen Kit
Primary Antibody Myeloperoxidase/MPO Antibody Picoband®
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Polymer Anti-Rabbit IgG–HRP Immunohistochemistry Kit
Detection Imaging system:Leica DM2500
Results Summary MPO (myeloperoxidase) is a lysosomal enzyme mainly present in neutrophils and monocytes/macrophages. Its core function is to generate reactive oxygen species, and therefore it plays a “double-edged sword” role in innate immune defense and inflammation-related diseases. MPO is present at very low levels in normal skin, but in burned skin, a large number of macrophages accumulate around the injured tissue, accompanied by a significant increase in MPO expression. Based on the immunohistochemical results, the staining is clear and the findings are consistent with expectations.

This antibody is highly specific and efficient, suitable for detecting STAT3 protein in rat colon by Western blot, with clear results.

Excellent, submitted by on
SKU M00007
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein STAT3 and the loading control Actin in the colon of normal rats, model rats, low/medium/high dose Chinese medicine treatment groups, and Western medicine treatment group. No differences were observed between the groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of STAT3 (Phospho-Y705) protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by on
SKU P00007-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of the target protein STAT3 (Phospho-Y705) and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of SLC6A4 protein in rat colon tissue, with only slight nonspecific bands observed.

Excellent, submitted by on
SKU PB9438
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Serotonin transporter/SLC6A4 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows representative Western blot results of the target protein SLC6A4 and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.