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The Anti-TH antibody (M01917) demonstrated excellent specificity and clear fluorescence staining in IF analysis of paraffin-embedded mouse striatum sections, accurately labeling dopaminergic neurons with minimal background.

Excellent, submitted by Zengting Li, Huazhong University of Science and Technology on 2026-02-12

SKU	M01917
Application	Immunofluorescence
Sample	mouse brain
Sample Processing Description	Sagittal sections of mouse striatum were fixed in formaldehyde for 48 hours and paraffin-embedded.
Other Reagents	Goat serum，DAPI，Anti-fade mounting medium
Primary Antibody	Iba1 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	1:500, Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	TH is a marker of catecholaminergic neurons and can specifically label and identify dopaminergic, noradrenergic, and adrenergic neurons. In this experiment, TH immunofluorescence was performed to observe the distribution of dopaminergic neurons in the striatum. The results show clear staining and precise localization.

Using IHC on paraffin-embedded human placental tissues, the Anti-Histone H3 (acetyl K27) antibody (M12477-15) showed high specificity and clear staining, with higher H3K27 acetylation in preterm placentas than in normal controls.

Excellent, submitted by Yuan Wang, Shandong First Medical University on 2026-02-04

SKU	M12477-15
Application	Immunohistochemistry
Sample	normal and preterm human placentas
Sample Processing Description	Clinically collected normal and preterm placentas were sectioned longitudinally (with amnion and chorion visible) and prepared as paraffin-embedded sections.
Other Reagents	Tris-EDTA Antigen Retrieval Solution, DAB Chromogenic Solution
Primary Antibody	Histone H3 (acetyl K27) Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Polymer anti-rabbit IgG-HRP IHC detection kit
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	Histone H3 (acetyl K27) is the acetylated form of histone H3 at lysine 27 and serves as a sensitive indicator of epigenetic abnormalities in placental cells; IHC results showed higher expression in preterm placentas than in normal placentas.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by David Lee on 2026-01-08

SKU	A01263
Application	Western Blot
Sample	Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description	Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody	Anti-beta Actin ACTB Antibody
Primary Incubation	1:1000, incubated overnight at 4°C.
Secondary Antibody	Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation	1:5000 dilution, 1 hour at room temperature.
Other Reagents used	5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection	Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary	Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

This antibody is highly specific and efficient, suitable for Western blot detection of C1QBP protein in mouse liver and brown adipose tissue, with no nonspecific bands observed. However, due to experimental conditions and the particular characteristics of

Excellent, submitted by Jiaqi Xin, Capital Medical University on 2026-01-08

SKU	M01439
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Anti-GC1q R Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows Western blot results of the target protein and the loading control in mouse liver and brown adipose tissue from normal and treated groups, with or without enzymatic digestion. The target bands in the liver are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting STAT3 protein in rat colon by Western blot, with clear results.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-08

SKU	M00007
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows Western blot results of the target protein STAT3 and the loading control Actin in the colon of normal rats, model rats, low/medium/high dose Chinese medicine treatment groups, and Western medicine treatment group. No differences were observed between the groups. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of STAT3 (Phospho-Y705) protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-07

SKU	P00007-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-STAT3 (Y705) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of the target protein STAT3 (Phospho-Y705) and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for detecting RHOA/B/C protein in rat colon by Western blot, with only minimal non-specific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00207-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Rho A + B + C Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein RHOA/B/C and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of PTEN protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00006-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	PTEN Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows a schematic of Western blot results for the target protein PTEN and the internal control Actin in rat colon across different groups. Expression was increased in the model group. Among the low, medium, and high doses of the herbal treatment, the low-dose group showed the best effect. The target bands are clear, and the experimental results are satisfactory.

This antibody is highly efficient and specific, suitable for Western blot detection of JAK2 protein in rat colon tissue, with only minor nonspecific bands.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00027
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	JAK2 Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein JAK2 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and well defined, and the experimental results are satisfactory.

This antibody exhibits high efficiency and specificity and is suitable for Western blot detection of CCND1 protein in rat colon tissue, with only minor nonspecific bands observed.

Excellent, submitted by Shiyu Zhang, LUTCM on 2026-01-06

SKU	M00149-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Cyclin D1 CCND1 Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows the Western blot results of the target protein CCND1 and the internal control Actin in rat colon tissue across the following groups: normal, disease model, low/middle/high dose traditional Chinese medicine treatment, and Western medicine treatment. The target bands are clear and distinct, and the experimental results are satisfactory.