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Western blot analysis using Anti-COL3A1 Antibody (M00788-1) showed clear and specific bands in mouse myocardium samples from 3-, 6-, 12-, and 18-month-old mice, with overall comparable COL3A1 expression and a slight, non-significant increase with age.

Excellent, submitted by Huanping Qian, Ningxia Medical University on 2026-03-05

SKU	M00788-1
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Myocardial tissues were collected from mice at 3, 6, 12, and 18 months of age, and total protein was extracted.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, blocking buffer
Primary Antibody	Collagen III Rabbit Monoclonal Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	This experiment aimed to study the changes in COL3A1 protein in mouse myocardium at 3, 6, 12, and 18 months of age (representing young, adult, middle-aged, and old mice). The results show that COL3A1 levels are largely similar across ages, with a slight, non-significant increase as age progresses.

Western blot using Anti-ER/ESR1 Antibody (M00057-2) in mouse brain tissues showed clear, specific ESR1 bands across normal, disease model, and AB drug-treated groups, with β‑actin as a consistent loading control.

Excellent, submitted by Yetao Ju, Liaoning University of Traditional Chinese Medicine on 2026-03-05

SKU	M00057-2
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4°C for 2 hours, centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer, denatured by heating at 95–100°C for 10 minutes, and 15 μL of protein was loaded per lane for SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	goat anti rabbit secondary antibodies
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

WB results showed that VEGFC (M00623) was upregulated by LPS and reduced after dexamethasone or homemade drug treatment, with clear and specific bands, indicating an anti-inflammatory effect.

Excellent, submitted by Kaitong Yang, Jiangnan University, Wuxi Medical College on 2026-03-04

SKU	M00623
Application	Western Blot
Sample	Human retinal microvascular endothelial cells
Sample Processing Description	Human retinal microvascular endothelial cells treated with LPS and then with dexamethasone or homemade drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer
Primary Antibody	VEGFC Antibody
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:10000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	VEGFC is an important signaling protein in vivo that primarily acts on lymphatic and blood vascular endothelial cells, promoting lymphangiogenesis and angiogenesis. WB results showed that VEGFC expression was increased in LPS-stimulated human retinal microvascular endothelial cells and decreased after treatment with dexamethasone or a homemade drug, indicating the anti-inflammatory effect of the homemade drug.

WB results showed that TPH1 (A01626-1) was upregulated in the rat colon model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-03-04

SKU	A01626-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	Rat colon tissues were lysed in RIPA buffer containing PMSF (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 min, and then loaded onto SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Tryptophan Hydroxylase/TPH1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows WB results of TPH1 and the internal control Actin in rat colon across different groups; expression was elevated in the model group and reduced after Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

WB results showed that CCNB1 (A00745-1) was upregulated in the rat colon model group and reduced after treatment, with clear and specific bands.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-03-04

SKU	A00745-1
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer containing PMSF (100:1) was used for lysis for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100°C for 10 min, and then subjected to SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Cyclin B1/CCNB1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows WB results of CCNB1 and the internal control Actin in rat colon across different groups; CCNB1 expression was elevated in the model group and decreased after traditional Chinese medicine treatment, with the high-dose group showing the best effect, and the target bands were clear and specific, indicating satisfactory results.

Anti-PRLR Antibody (PA2087) demonstrated clear and specific detection of PRLR in mouse brain tissue by Western blot, with distinct differences observed among the control, model, and AB treatment groups.

Excellent, submitted by Yetao Ju, Liaoning University of Traditional Chinese Medicine on 2026-02-28

SKU	PA2087
Application	Western Blot
Sample	mouse brain tissue
Sample Processing Description	Mouse brain tissues were lysed in RIPA buffer containing a protease inhibitor cocktail at 4 °C for 2 hours. After centrifugation, the supernatant was collected for protein quantification. The protein concentration was adjusted accordingly, mixed with 5× protein loading buffer, and denatured by heating for 10 minutes. Then, 15 μl of protein sample was loaded per lane for electrophoresis.
Other Reagents	blocking buffer
Primary Antibody	Prolactin Receptor/PRLR Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)
Secondary Incubation	1:2000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of PRLR and the internal control β-actin in brain tissues from normal mice, the model group, and mice treated with low and high doses of AB. The antibody produced clear bands, and distinct differences among the experimental groups were clearly observed.

Immunofluorescence analysis using the Anti-CD68 antibody (A00602-1) on human normal and preterm placenta showed minimal CD68 expression in normal samples and abundant expression in preterm inflamed samples, consistent with expected results.

Excellent, submitted by Yuan Wang, Shandong First Medical University on 2026-02-27

SKU	A00602-1
Application	Immunofluorescence
Sample	human normal and preterm placenta
Sample Processing Description	Human normal-term and preterm placentas were collected clinically and processed for longitudinal paraffin embedding, allowing visualization of both the amnion and chorion membranes.
Other Reagents	Goat serum, DAPI, anti-fade mounting medium
Primary Antibody	Anti-CD68 Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)（BA1148）
Secondary Incubation	1:500, 45 min in 37℃
Detection	Image system:Leica DM2500
Results Summary	In normal placentas, macrophages are scarce or nearly absent, showing only sporadic CD68 expression, whereas in preterm placentas with inflammation, macrophages are abundant and CD68 expression is markedly increased, consistent with expected theoretical data.

Western blot of OCI-LY1 cells treated with SPHINX31 showed stable GAPDH expression with clear bands and clean background, demonstrating high specificity of A00227-1.

Excellent, submitted by Maolin Yao, Fujian Medical University on 2026-02-27

SKU	A00227-1
Application	Western Blot
Sample	human OCI-LY1 cellls
Sample Processing Description	Cell samples were lysed by sonication in RIPA buffer containing protease and phosphatase inhibitors, followed by centrifugation for 10 minutes. The supernatant was mixed with loading buffer at a 4:1 ratio, boiled for 10 minutes, and 15 μL of protein was loaded per well.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:5000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	In OCI-LY1 cells treated with different concentrations of SPHINX31 for 24 h, GAPDH expression remained stable with no significant differences, showing clear bands and a clean background without nonspecific signals.

The Anti-GAPDH antibody (A00227-1) demonstrated high sensitivity and clear WB bands in mouse intestinal tissue, offering excellent cost-effectiveness and reliability, and is highly recommended for use.

Excellent, submitted by Qihang Hou, China Agricultural University on 2026-02-27

SKU	A00227-1
Application	Western Blot
Sample	mouse intesinal tissue
Sample Processing Description	Mouse colon tissue was lysed in RIPA buffer containing a protease inhibitor cocktail. Protein concentration was determined using the Pierce™ BCA Protein Assay Kit, and equal amounts of protein were loaded after boiling denaturation.
Other Reagents	5% non-fat milk
Primary Antibody	GAPDH Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	goat anti rabbit secondary antibodies
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Image system:ChemiDoc MP
Results Summary	This antibody is highly sensitive, produces clear WB bands, is reusable, offers excellent cost-effectiveness, and demonstrates a clear advantage over similar international products, making it highly recommended for use.

Using Boster’s IL-6 antibody (RP1012) in WB on HK-2 cells produced clear, specific bands with high sensitivity and cost-effectiveness, outperforming previously tested antibodies.

Excellent, submitted by Jingwen Zhang, Ocean University of China on 2026-02-27

SKU	RP1012
Application	Western Blot
Sample	human HK-2 cells
Sample Processing Description	Cell samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured at 98 °C. Twenty microliters of each protein sample were loaded per lane onto SDS-PAGE.
Other Reagents	5% non-fat milk
Primary Antibody	Interleukin-6 IL6 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate
Results Summary	Previously, WB experiments were performed using antibodies from two domestic and international suppliers, which showed poor specificity and were expensive. Subsequently, Boster’s IL-6 antibody (Cat. RP1012) was used, demonstrating high specificity, strong titer, cost-effectiveness, and yielding clear bands.