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Pancreatic IL-6 levels measured using the Boster Anti-IL-6 antibody (PB9034) demonstrated high specificity and sensitivity in WB analysis, providing reliable evidence for evaluating the anti-inflammatory effect of the nanodrug.

Excellent, submitted by Jiahui Yan, College of Chemistry and Chemical Engineering, Ocean University of China on 2026-02-27

SKU	PB9034
Application	Western Blot
Sample	mouse PACs cells
Sample Processing Description	After centrifugation, the supernatant was collected. A small aliquot of the protein solution was taken for BCA quantification, and the remaining protein solution was mixed with an equal volume of loading buffer, then denatured in a 100 °C water bath for 5 minutes.
Other Reagents	Protein-Free Rapid Blocking Buffer
Primary Antibody	Anti-IL6 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1 h in RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	The levels of IL-6 and IL-1β in the pancreas are key indicators for evaluating whether the nanodrug exerts anti-inflammatory effects. Initially, antibodies from two domestic and international suppliers were used for WB experiments, but their specificity was relatively poor. Subsequently, the antibody from Boster was adopted, which demonstrated strong specificity, high titer, and cost-effectiveness.

In WB using Anti-CDK1 (Phospho-T450) antibody (Cat# PB9533-50 µL), CDK1 expression in rat colon was increased in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and well-defined target bands.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-02-26

SKU	PB9533
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	Samples were lysed in RIPA buffer containing PMSF protease inhibitor (100:1) for 10 min, centrifuged at 12,000 rpm for 15 min, and the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents	5% Non-fat milk
Primary Antibody	CDK1 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The image shows WB results of CDK1 and the loading control Actin in rat colon across different groups; CDK1 expression was elevated in the model group and most effectively reduced in the high-dose herbal treatment group, with clear and distinct target bands.

In WB using Occludin antibody (Cat# A01246), clear bands showed decreased Occludin in the model group and best recovery in the high-dose herbal treatment group.

Excellent, submitted by Shiyu Zhang, Liaoning University of Traditional Chinese Medicine on 2026-02-26

SKU	A01246
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer containing protease inhibitor PMSF (100:1) was used to lyse samples for 10 min, followed by centrifugation at 12,000 rpm for 15 min; the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE.
Other Reagents	5% Non-fat milk
Primary Antibody	Occludin OCLN Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)
Secondary Incubation	1:5000, 1 h in RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	The image shows WB results of Occludin and the loading control Actin in rat colon across different groups; Occludin expression was reduced in the model group, with the high-dose herbal treatment showing the best recovery and clear, well-defined target bands.

In IHC using Ki67 antibody (Cat# PB9026), strong and specific nuclear staining was observed in nude mouse tumor tissue, confirming high Ki67 expression and active cell proliferation.

Excellent, submitted by Jin Sun, Tangdu Hospital, Xi’an, China on 2026-02-26

SKU	PB9026
Application	Western Blot
Sample	Nude mouse tumor tissue
Sample Processing Description	Paraffin-embedded tumor tissue sections.
Primary Antibody	Ki67/MKI67 Antibody Picoband®
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation	1 hour in room temperature
Detection	Substrate: ECL reagent, Imaging system:ChemiDoc MP
Results Summary	This antibody is highly specific and efficient, with a clean background and no nonspecific bands. The target band has sharp and well-defined edges.

In this IHC experiment using Anti-SLC22A3 antibody (Cat# M04914), strong and clear expression was observed in cervical cancer tumor cells, with further comparison planned between tumor and adjacent tissues.

Excellent, submitted by Juan Wang, The First Affiliated Hospital of Shihezi University on 2026-02-25

SKU	M04067-2
Application	Immunohistochemistry
Sample	human cervical cancer tissue
Sample Processing Description	Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other Reagents	Goat serum, DAB substrate solution
Primary Antibody	SLC22A3 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Two-step IHC detection kit
Secondary Incubation	30 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	IHC using SLC22A3 antibody (M04914) showed strong and clear expression in tumor cells of human cervical carcinoma, and the next step will be to compare expression between tumor and adjacent normal tissue.

In this IHC experiment using Anti-SERPINB3 antibody (Cat# M04067-2) on human cervical cancer paraffin sections, SERPINB3 was highly expressed in tumor cells with clear positive staining and low background, demonstrating excellent antibody performance.

Excellent, submitted by Juan Wang, The First Affiliated Hospital of Shihez on 2026-02-25

SKU	M04067-2
Application	Immunohistochemistry
Sample	human cervical cancer tissue
Sample Processing Description	Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other Reagents	Goat serum, DAB substrate solution
Primary Antibody	SerpinB3 Rabbit Monoclonal Antibody
Primary Incubation	1:400, overnight at 4 ℃
Secondary Antibody	Two-step IHC detection kit
Secondary Incubation	30 min at 37℃
Detection	Imaging system:Leica DM2500
Results Summary	The results demonstrated high expression of SERPINB3 in cervical cancer tumor cells with clear positive staining; further studies will compare its expression levels between tumor and adjacent non-tumor tissues.

In this IHC experiment using Anti-CD4 antibody (Cat# M00344) on human cervical cancer paraffin sections, CD4-positive helper T cells were clearly and specifically stained with minimal background, demonstrating excellent antibody performance.

Excellent, submitted by Yanan Peng, The First Affiliated Hospital of Shihezi University on 2026-02-25

SKU	M00344
Application	Western blot
Sample	human cervical cancer paraffin sections
Sample Processing Description	Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded
Other Reagents	Goat serum, DAB substrate solution
Primary Antibody	CD4 Rabbit Monoclonal Antibody
Primary Incubation	1:200, overnight at 4 ℃
Secondary Antibody	Two-step IHC detection kit
Secondary Incubation	30 min at 37℃
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	CD4 is a marker of helper T cells; in this IHC experiment, CD4 staining clearly identified helper T cells in human cervical cancer samples, showing excellent specificity and performance of the antibody.

In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	M00865
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	AQP1 Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at RT
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

In this WB experiment using Anti-CAMP antibody (Cat# A05475-1) on MADB106 rat mammary carcinoma cells, CAMP protein secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	A05475-1
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	Camp Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at 37℃
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	CAMP, also known as cathelicidin antimicrobial peptide or LL-37, is an important antimicrobial peptide with dual functions: directly killing pathogens and modulating immune responses. It plays a complex role in maintaining the homeostasis of barrier tissues such as skin and in influencing tumor progression. In breast cancer, CAMP may promote tumor development by enhancing angiogenesis and cancer cell proliferation. In this experiment, CAMP secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.

In this Western blot (WB) experiment using Anti-AQP5 antibody (Cat# A03085) on MADB106 rat mammary carcinoma cells, AQP5 protein levels were significantly increased with agonist treatment and markedly reduced with inhibitor treatment.

Excellent, submitted by Xinshuo Wang, Xi’an Jiaotong University on 2026-02-25

SKU	A03085
Application	Western blot
Sample	MADB106 rat mammary carcinoma cells
Sample Processing Description	MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor
Other Reagents	RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer
Primary Antibody	Aquaporin 5/AQP5 Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	1:10,000, HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation	1h at 37℃
Detection	Substrate: ECL substrate, Imaging system:ChemiDoc MP
Results Summary	AQP5 (Aquaporin 5) is a transmembrane water channel protein that primarily regulates cellular water transport and plays a critical and complex role in tumor progression. In breast cancer, high AQP5 expression is often associated with increased tumor invasiveness, lymph node metastasis, and poor patient prognosis. In this experiment, AQP5 protein levels were markedly increased in cells treated with the agonist, whereas they were significantly reduced in cells treated with the inhibitor.