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In an immunofluorescence experiment using normally cultured Caco-2 cells, the ZO-1 antibody (PB9234) showed clear and specific membrane staining with low background, demonstrating reliable performance.

Excellent, submitted by on
SKU PB9234
Application Immunofluorescence
Sample human Caco-2 cell line
Sample Processing Description Cells were normally cultured in 24-well plates using MEM supplemented with 20% fetal bovine serum. When the cell density reached approximately 60%, the culture was terminated. The medium was removed, cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and then washed three times with PBS before further use.
Other ReagentsGoat serum, DAPI, and an anti-fade mounting medium.
Primary Antibody ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Caco-2 cells are a “gold standard” in vitro model for studying intestinal epithelial function, and ZO-1 is a key marker of barrier integrity. The images show immunofluorescence staining of ZO-1 in normally cultured Caco-2 cells to evaluate antibody performance. Clear membrane localization and accurate staining indicate that this antibody is suitable for downstream research applications.

Using IHC on paraffin-embedded human placental tissues, the Anti-Histone H3 (acetyl K27) antibody (M12477-15) showed high specificity and clear staining, with higher H3K27 acetylation in preterm placentas than in normal controls.

Excellent, submitted by on
SKU M12477-15
Application Immunohistochemistry
Sample normal and preterm human placentas
Sample Processing Description Clinically collected normal and preterm placentas were sectioned longitudinally (with amnion and chorion visible) and prepared as paraffin-embedded sections.
Other ReagentsTris-EDTA Antigen Retrieval Solution, DAB Chromogenic Solution
Primary Antibody Histone H3 (acetyl K27) Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Polymer anti-rabbit IgG-HRP IHC detection kit
Secondary Incubation 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary Histone H3 (acetyl K27) is the acetylated form of histone H3 at lysine 27 and serves as a sensitive indicator of epigenetic abnormalities in placental cells; IHC results showed higher expression in preterm placentas than in normal placentas.

IF using Anti-CD31 antibody (A01513-2) showed specific, strong, and stable endothelial staining, revealing expanded and inflamed microvessels in treated mouse skin compared to controls.

Excellent, submitted by on
SKU A01513-2
Application Immunofluorescence
Sample Paraffin-embedded mouse skin sections
Sample Processing Description Mouse skin samples were divided into three groups: (i) normal control, (ii) congestion group, treated with ethanol, and (iii) inflammation group, stimulated with LPS.
Other Reagents Goat serum, DAPI, Anti-fade mounting medium
Primary Antibody CD31/Pecam1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody DyLight 488–conjugated Goat Anti-Rabbit IgG (H+L)) (Boster, BA1127)
Secondary Incubation 1:500, 45 min at 37℃
Detection Imaging system:Leica DM2500
Results Summary IF staining with Boster CD31 antibody produced high-quality vascular images. In the control group, vessels were small and well-defined. In the congestion group (ethanol-treated), CD31 staining revealed markedly dilated vascular lumens. In the inflammation group (LPS-treated), vessel dilation was accompanied by changes in tissue spacing. Combined with Prussian blue staining and magnetic signal measurements, we observed that despite extreme vessel dilation in the congestion group, magnetic signals were not significantly elevated; only the inflammation group, with extensive macrophage infiltration and uptake, showed high magnetic signals.

Immunofluorescence using Anti-GFAP antibody (MA1045) clearly labeled protoplasmic astrocytes in the spinal cord gray matter, showing dense, bushy processes surrounding neurons, with excellent specificity and staining.

Excellent, submitted by on
SKU MA1045
Application Immunofluorescence
Sample rat spinal tissue
Sample Processing Description Paraffin-embedded transverse sections of rat spinal cord were prepared after formalin fixation.
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAPI
Primary Antibody GFAP Antibody (Monoclonal, G-A-5)
Primary Incubation 1:200, overnight at 4 ℃
Secondary Antibody Goat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 Conjugated
Secondary Incubation 45 minutes in 37℃
Detection Imaging system:Leica DM2500
Results Summary GFAP is a marker of astrocytes. In this experiment, immunostaining for GFAP was used to label astrocytes in the gray matter of the spinal cord to observe their distribution, density, and morphology. The results showed that the labeled protoplasmic astrocytes in the gray matter had short, thick, and highly branched processes with rough surfaces, forming a dense “bushy” network tightly surrounding neuronal cell bodies and synapses, consistent with theoretical expectations and demonstrating excellent staining.

IHC using Tyrosine Hydroxylase/TH Antibody Picoband® (P00683) showed clear staining with no background, revealing markedly reduced TH expression in the medulla of Alzheimer’s mouse brain compared to normal mouse brain.

Excellent, submitted by on
SKU PB9449
Application Immunohistochemistry
Sample Normal mouse brain and Alzheimer’s model mouse brain tissue
Sample Processing Description Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain.
Other Reagents Goat serum, DAB
Primary Antibody Tyrosine Hydroxylase/TH Antibody Picoband®
Primary Incubation 1:500, overnight at 4 ℃
Secondary Antibody Two-step IHC kit
Secondary Incubation 37 minutes in 37 ℃
Detection Imaging system:Leica DM2500
Results Summary TH is a marker of catecholaminergic neurons, specifically labeling dopaminergic, noradrenergic, and adrenergic neurons. IHC results showed that TH expression in the medulla was markedly lower in Alzheimer’s mouse compared to normal mouse.

IHC with HDAC5 Antibody Picoband® (A01230-6) showed clear staining and low background, with significantly higher HDAC5 levels in the cortex of Alzheimer’s mice, suggesting potential as a biomarker.

Excellent, submitted by on
SKU A01230-6
Application Immunohistochemistry
Sample Normal mouse brain and Alzheimer’s model mouse brain
Sample Processing Description Paraffin-embedded normal mouse brain and Alzheimer’s model mouse brain.
Other Reagents Goat serum, DAB chromogen
Primary Antibody Anti-HDAC5 Antibody Picoband®
Primary Incubation 1:400, overnight at 4 ℃
Secondary Antibody Two-step IHC kit
Secondary Incubation 30 minutes in 37 ℃
Detection Imaging system: Leica DM2500
Results Summary The results showed that HDAC5 levels in the cortex were significantly higher in Alzheimer’s mice than in normal mice, suggesting its potential as a biomarker for further validation.

Using Myeloperoxidase/MPO Antibody Picoband® (PA1054) in IHC, MPO showed low expression in normal mouse skin and was markedly increased in burned skin, with clear staining and expected results.

Excellent, submitted by on
SKU PA1054
Application Immunohistochemistry
Sample mouse skin tissue
Sample Processing Description Male BALB/c mice aged 6–8 weeks were used. (1) Normal dorsal skin was collected from untreated mice. (2) Burn injury was induced on the dorsal skin using a burn device. After one week of housing, hair was removed with depilatory cream. Skin samples were collected from normal mice (dorsal skin) and burned mice (burned area), fixed in formalin for 72 hours, and embedded in paraffin for sectioning./td>
Other ReagentsTris-EDTA Antigen Retrieval Buffer (50×, pH 9.0), DAB Chromogen Kit
Primary Antibody Myeloperoxidase/MPO Antibody Picoband®
Primary Incubation 1:100, overnight at 4 ℃
Secondary Antibody Polymer Anti-Rabbit IgG–HRP Immunohistochemistry Kit
Detection Imaging system:Leica DM2500
Results Summary MPO (myeloperoxidase) is a lysosomal enzyme mainly present in neutrophils and monocytes/macrophages. Its core function is to generate reactive oxygen species, and therefore it plays a “double-edged sword” role in innate immune defense and inflammation-related diseases. MPO is present at very low levels in normal skin, but in burned skin, a large number of macrophages accumulate around the injured tissue, accompanied by a significant increase in MPO expression. Based on the immunohistochemical results, the staining is clear and the findings are consistent with expectations.

Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

Excellent, submitted by on
SKU A01263
Application Western Blot
Sample Human 293T and A549 cell lysates, Mouse brain tissue
Sample Processing Description Cells were lysed in RIPA buffer containing protease inhibitors. Lysates were clarified by centrifugation and quantified using a BCA assay. 30–35 µg of protein was loaded per lane on a 5–20% SDS-PAGE gel and transferred to nitrocellulose membranes.
Primary Antibody Anti-beta Actin ACTB Antibody
Primary Incubation 1:1000, incubated overnight at 4°C.
Secondary Antibody Goat anti-rabbit IgG-HRP (Catalog # BA1054).
Secondary Incubation 1:5000 dilution, 1 hour at room temperature.
Other Reagents used 5% non-fat milk/TBST blocking buffer, ECL Plus substrate, Azure Biosystems c600 imaging system.
Detection Chemiluminescent detection. Strong single band observed at ~42 kDa, corresponding to beta actin.
Results Summary Produced clear, sharp bands with no background. Excellent specificity and consistency across replicates. Ideal as a loading control for WB.

This antibody is highly specific and efficient, suitable for Western blot detection of C1QBP protein in mouse liver and brown adipose tissue, with no nonspecific bands observed. However, due to experimental conditions and the particular characteristics of

Excellent, submitted by on
SKU M01439
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody Anti-GC1q R Rabbit Monoclonal Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein and the loading control in mouse liver and brown adipose tissue from normal and treated groups, with or without enzymatic digestion. The target bands in the liver are clear and well defined, and the experimental results are satisfactory.

This antibody is highly specific and efficient, suitable for Western blot detection of TTF2 protein in rat colon, with clear bands.

Excellent, submitted by on
SKU A07013-2
Application Western Blot
Sample rat colon tissue
Sample Processing Description RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE.
Other Reagents Blocking buffer
Primary Antibody TFF2 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation 1:5000, 1 hour in room temperature
Detection Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary The figure shows Western blot results of the target protein TTF2 and the internal control Actin in rat colon from the normal control group, model group, low/medium/high-dose traditional Chinese medicine treatment groups, and the Western medicine treatment group. The target bands are clear and well defined, and the experimental results are satisfactory.