| SKU | M00220-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse lung cancer tissue |
| Sample Processing Description | Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE. |
| Primary Incubation | The membrane was incubated with the CD86 primary antibodies (1:2000) overnight at 4 °C. |
| Secondary Antibody | HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | 5% non-fat milk |
| Detection | Signal was developed using ECL substrate on an Tanon system. |
| Results Summary | The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands. I will definitely continue using BosterBio products and will recommend them to my classmates and colleagues. |
