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IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective.

Excellent, submitted by on
SKU M00101-1
Application Western Blot
Sample Mouse pancreatic acinar cells
Sample Processing Description After centrifugation, collect the supernatant. Take a small portion for protein quantification using the BCA assay. Mix the remaining protein solution with an equal volume of loading buffer and denature in a 100°C water bath for 5 mins.
Primary Incubation The membrane was incubated with the IL-6 and IL-1β primary antibodies (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Protein-Free Blocking Buffer
Detection Signal was developed using ECL substrate on a ChemiDoc MP system.
Results Summary IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective. In our subsequent experiments, our group continued to use BosterBio products, which proved to be highly reliable and provided solid support for the publication of several articles.

Works very well in Western blot for detecting pChk2 in human HeLa cells.

Excellent, submitted by on
SKU P00277-1
Application Western Blot
Sample Hela cell
Sample Processing Description After 12 h of viral infection, cells were lysed in RIPA buffer with PMSF and heated in 1× protein loading buffer for 10 min to denature proteins.
Primary Incubation The membrane was incubated with the CHEK2 (Phospho-T68) primary antibody (1:2000) overnight at 3 hours.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used None
Detection Signal was developed using ECL substrate on an ChemiDoc MP system.
Results Summary Works very well in Western blot for detecting pChk2 in human HeLa cells.

Anti-phospho Lyn (Y396) Rabbit Monoclonal Antibody

Excellent, submitted by on

Source: Biocompare.com

SKU P01424-1
Application Flow Cytometry
Sample B cells
Primary Incubation 0.25 µl/10^6 cells 50min RT
Blocking Agent FBS 5%
Secondary Incubation F(ab')2-Donkey anti-Rabbit IgG (H+L), PE 0.1 µl/10^6
Detection Flow Cytometry
Results Summary Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million cells with pLYN antibody (P01424-1) during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody only and in blue primary anti-phospho lyn + secondary antibody.

Good GATA3 Antibody from BosterBio

--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent, submitted by on

Source: Biocompare.com

SKU M00593
Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Immunohistochemistry for Anti-Cyclophilin B/PPIB Antibody

Excellent, submitted by on
SKU A03229
Application Immunohistochemistry (paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Placenta
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 37°C for 30 minutes
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 0.5ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation at 37°C for 30 min