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Western blot analysis of AKT1 protein in mouse hippocampus using the AKT1 antibody showed clear bands. The antibody is suitable for mouse hippocampal tissue samples.

Excellent, submitted by on
SKU M00024-1
Application Western Blot
Sample Mouse hippocampus tissue
Sample Processing Description The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE.
Primary Antibody Anti-AKT1 Monoclonal Antibody
Primary Incubation overnight at 4 ℃
Secondary Antibody HRP-conjugated Anti-Rabbit IgG Secondary Antibody
Secondary Incubation 1 hour in room temperature
Detection Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system: ChemiDoc MP (Bio-Rad)
Results Summary Western blot analysis of AKT1 protein in the mouse hippocampus using the AKT1 antibody showed clear bands. This antibody is suitable for mouse hippocampal tissue samples.

The staining is clear, highly specific, with almost no non-specific bands.

Excellent, submitted by on
SKU PA2290
Application Western Blot
Sample Protein extracts from LA795 and 16HBE cells.
Sample Processing Description After collecting the cells, lyse them with RIPA buffer containing protease inhibitors, quantify the protein using the BCA assay, and add loading buffer proportionally. Denature by heating at 98 °C. Load the samples onto SDS-PAGE, with 30 μg of total protein per lane.
Primary Antibody Anti-TMEM16A/ANO1 Antibody Picoband®
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent 5% non-fat milk
Secondary Antibody HRP-conjugated goat anti-rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Substrate: ECL substrate, Imaging system: Tanon
Results Summary The TMEM16A antibody we previously used was never ideal; despite long-term optimization, it was difficult to obtain clear bands. This time, by using BOSTER’s polyclonal antibody, we achieved much better experimental results. The bands were successfully exposed in essentially one attempt, with clear signals and no non-specific bands. We are very satisfied with Boster’s antibody.

I was so thrilled that I took the photo below — it felt like a true treasure.

Excellent, submitted by on
SKU PB9234
Application ICC/IF
Sample Endothelial cells
Sample Processing Description Endothelial cells were seeded in collagen gel-treated chips for 12 hours.
Primary Antibody Anti-ZO1 tight junction protein/TJP1 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Ready-to-use Goat Serum
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, DyLight®488 Conjugated(BA1127)
Secondary Incubation Incubate at room temperature for 1 hour
Other reagents DAPI (AR1176)， Anti-fade mounting medium
Detection Fluorescence microscope
Results Summary At a critical stage of our manuscript preparation, we needed to stain tight junction proteins in endothelial cells. After trying primary antibodies from many companies without achieving satisfactory results, it was ultimately Boster’s product that solved the problem (Figure 5A). I was so excited that I took the photo below — treating it like a true treasure.

In this study, these two antibodies were primarily used for Western blot experiments. In the Western blot results, the CCT1 antibody showed a strong specific band and maintained a robust signal even after multiple reuses.

Excellent, submitted by on
SKU M02389
Application Western Blot
Sample U2OS cell
Sample Processing Description Cells were lysed with NP-40 lysis buffer to extract proteins. After quantification using the BCA assay, 5× loading buffer was added, and the samples were boiled for denaturation. Then, 20 µg of protein was loaded into each lane.
Primary Antibody Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7)
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent BSA
Secondary Antibody Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Unconjugated (BA1039)
Secondary Incubation Incubate at room temperature for 1 hour
Detection Substrate: ECL substrate, Imaging system: ChemiDoc MP (Bio-Rad)
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

Highly prone to positive signals, with precise localization of expression

Excellent, submitted by on
MA1083 Immunofluorenscence
SKU MA1083
Application Immunofluorenscence
Sample Mouse MC-8 cells
Sample Processing Description 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-PCNA Antibody (Monoclonal, PC 10)
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

Highly prone to positive signals, with precise expression localization

Excellent, submitted by on
SKU M00656
Application Immunofluorenscence
Sample Mouse MC-8 cells
Sample Processing Description 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 488-conjugated Goat Anti-Rabbit IgG.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

BOSTER’s rabbit anti-MBP antibody (catalog BA0094) exhibits high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

Excellent, submitted by on
SKU PA1050
Application Immunofluorenscence
Sample Mouse spinal cord
Sample Processing Description Mouse spinal cord fixed with 2% paraformaldehyde for 6–8 hours, dehydrated in 30% sucrose, embedded in OCT, and sectioned using a cryostat.
Primary Antibody Anti-MBP9 antibody
Primary Incubation 1:100, overnight at 4 ℃
Blocking Agent 3% BSA
Secondary Antibody Anti-rabbit IgG-CY3 conjugated antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Immunofluorescence images were acquired using a confocal laser microscope (Leica SP8, Zeiss LSM 880 Airyscan).
Results Summary BOSTER’s rabbit anti-MBP antibody (catalog BA0094) has high specificity and low background, enabling sensitive detection of demyelination and myelin regeneration processes, greatly facilitating this study.

BOSTER’s Ki-67 antibody can effectively mark the proliferative activity of tumor cells. After treatment, a significant decrease in Ki-67 expression in tumor tissues can be clearly observed, indicating that the proliferative activity of tumor cells is mark

Excellent, submitted by on
SKU PB9026
Application Immunofluorenscence
Sample Cells in nude mouse tumor tissue
Sample Processing Description Sections of nude mouse tumor tissues fixed and embedded under different treatment conditions (Scale Bar = 100 μm)
Primary Antibody Anti-Ki67/MKI67 Antibody Picoband®
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 488-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Fluorescence microscope
Results Summary The antibodies used in the experiment demonstrated good sensitivity and high cost-effectiveness, providing strong support for the smooth progress of the study.

Accurate localization and good imaging quality

Excellent, submitted by on
SKU PB9640
Application Immunofluorenscence
Sample Mouse 4T1 cell climbing slides
Sample Processing Description Mouse 4T1 cells fixed with 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-GRP78 antibody
Primary Incubation 1:400, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary The ordering and delivery cycle of the antibodies is short — I was able to receive the products within a week, which saved a lot of time. The pre-sales and after-sales services are convenient and efficient. Since my background is in chemistry, I consulted Boster’s technical staff about many details of biological experiments. The technical specialists were patient and helpful in their analysis and explanations, which allowed me to avoid many detours during the actual experimental procedures. Most importantly, these antibodies offer a high cost-performance ratio, with good binding performance, excellent imaging results, and a high experimental success rate, providing a solid foundation for conducting related experiments.

Accurate localization and good imaging quality

Excellent, submitted by on
SKU PA1239
Application Immunofluorenscence
Sample Rat Brain
Sample Processing Description Fixed in 4% paraformaldehyde for 48 hours, followed by paraffin embedding and sectioning.
Primary Antibody Anti-GFAP antibody
Primary Incubation 1:500, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary During the experiment, this primary antibody can be conveniently used with the complimentary antibody diluent provided. The results obtained showed good quality and reproducibility, with no false-positive signals. Overall, this primary antibody offers excellent cost performance and provides key data and strong support for publication.