
| SKU | M00220-1 |
|---|---|
| Application | Western Blot |
| Sample | mouse lung cancer tissue |
| Sample Processing Description | Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Twenty microliters of each protein sample were loaded per lane onto an SDS-PAGE gel. |
| Other Reagents | 5% Non-fat milk |
| Primary Antibody | CD86/B7 2 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL reagent, Imaging system:Tanon |
| Results Summary | This antibody is highly specific and efficient, with a clear target band and no nonspecific bands. |










