• Compare Products

  • Table of Contents

Customer Testimonials

Peer Reviews from Real Labs

Browse peer-submitted results linked to specific products—ratings, images, and practical notes included. Contribute your own result to strengthen the evidence base and claim rewards after verification.

Share Your Review Browse Reviews ↓

Video Interviews

See real workflows and decision points—what researchers tried, what worked, and why. Share your story in a short interview and claim rewards!

Get Interview Rewards

Filter by category, reactivity, and host to find the most relevant peer results.

A strong nuclear signal for Cyclin E was observed in proliferating cells by IF. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Immunofluorescence
Sample Human HaCaT cell
Sample Processing Description Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked in 1% BSA for 1 hour before incubation with the primary antibody.
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:100-1:200, overnight at 4 ℃
Secondary Antibody Goat anti-rabbit IgG conjugated to Alexa Fluor 488
Secondary Incubation 1:1000, 1-2 hours in room temperature
Other Reagents used PBS, 0.1% Triton X-100, 1% BSA, DAPI for nuclear counterstaining
Detection Fluorescence microscopy using AlexaFluor488 (excitation 488 nm, emission 519 nm) using Zeiss LSM 900 Confocal Microscope with Airyscan 2
Results Summary The CyclinE-N15 antibody (DZ41310) worked fine in IF application using human cell lines. In IF, the nuclear localization of Cyclin E matched the known expression pattern. A fluorescent secondary antibody (Alexa Fluor 488) was used for detection and gave clear signal. Overall, a reliable antibody for D15 transcript Cyclin E detection in human cell systems.

Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

Excellent, submitted by on
SKU DZ41310
Application Western Blot
Sample Human HaCaT cell, Human A2780 cell, Human HCT cell
Sample Processing Description Dissection, Homogenization, Sample boiling in 1X Lamelli, SDS-PAGE, Standard Western Blotting
Primary Antibody Human CCNE1 Antibody
Primary Incubation 1:1000, overnight at 4 ℃
Secondary Antibody Anti-rabbit/mouse IgG horseradish peroxidase-conjugated
Secondary Incubation 1:10000, 1-2 hours in room temperature
Detection Biorad Chemidoc
Results Summary Using freshly prepared blocking buffer helped reduce background. Results were reproducible and matched the expected patterns.

Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Excellent, submitted by on
SKU DZ41119
Application Immunofluorescence
Sample Zebrafish retinal cryo-section
Sample Processing Description Embryos fixed in 4% PFA for 4h. Embryos washed in PBST and 30% then 50% sucrose. Embedded in OCT and cryo-sectioned at 20nm
Primary Antibody Zebrafish Tfap2a Antibody
Primary Incubation 1:100, overnight at 4 ℃
Detection Used a Nikon C2+ confocal microscope
Results Summary Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf.

Highly prone to positive signals, with precise expression localization

Excellent, submitted by on
SKU M00656
Application Immunofluorenscence
Sample Mouse MC-8 cells
Sample Processing Description 4% paraformaldehyde for 15 minutes
Primary Antibody Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
Primary Incubation 1:200, overnight at 4 ℃
Blocking Agent Goat serum
Secondary Antibody DyLight 488-conjugated Goat Anti-Rabbit IgG.
Secondary Incubation Incubate at room temperature for 1 hour
Detection Laser confocal microscopy
Results Summary I will purchase Boster products again and recommend them to my classmates and colleagues.

The antibody is highly efficient and specific, showing a clear target band with no non-specific bands.

Excellent, submitted by on
SKU M30929
Application Western Blot
Sample HEK293T
Primary Incubation 1:1000, overnight at 4 ℃
Blocking Agent 5% non-fat milk
Secondary Antibody Anti-Rabbit IgG Secondary Antibody, HRP-conjugated
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Azure Biosystems c600.
Results Summary I will definitely purchase BosterBio products again and recommend them to my classmates and colleagues.

Thank you for all the work on this project

Excellent, submitted by on
SKU DZ01481
Application Immunofluorescence
Sample wildtype and KO mutant zebrafish retina
Primary Incubation 1:200
Blocking Agent 10% normal goat serum and 2% bovine serum albumin in PBS
Secondary Antibody Alexa Fluor 568 goat anti-rabbit was used in blocking solution.

The antibody performs efficiently and specifically, with very few nonspecific bands.

Excellent, submitted by on
WB result image
SKU A03213-2
Application Western Blot
Sample MCF-7 cell
Sample Processing Description Cells were directly lysed in NP-40 buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Then, 20 µL of protein sample was loaded per lane onto SDS-PAGE.
Primary Antibody UBA6 Antibody
Primary Incubation 1:1000, overnight at 4 °C
Blocking Agent 5% Non-fat milk
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG
Secondary Incubation Incubate at room temperature for 1 hour
Detection Signal was developed using ECL substrate on a Tanon system.
Results Summary The antibody performs efficiently and specifically, with very few nonspecific bands.

This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.

Excellent, submitted by on
SKU M03918
Application Western Blot
Sample Mouse brown adipose tissue
Sample Processing Description Tissue and cell proteins were extracted using RIPA buffer. After BCA quantification, 5× loading buffer was added, and the samples were boiled for 5 minutes for denaturation.
Primary Incubation The membrane was incubated with the PLIN1 primary antibodies (1:1000) overnight at 4 °C.
Secondary Antibody Goat Anti-Rabbit IgG Antibody (1:5000)
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Non-fat milk
Detection Signal was developed using ECL substrate
Results Summary This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.

The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands.

Excellent, submitted by on
SKU M00220-1
Application Western Blot
Sample Mouse lung cancer tissue
Sample Processing Description Tissue samples were directly lysed in RIPA buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98 °C. Load 20 µL of protein sample per lane onto SDS-PAGE.
Primary Incubation The membrane was incubated with the CD86 primary antibodies (1:2000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used 5% non-fat milk
Detection Signal was developed using ECL substrate on an Tanon system.
Results Summary The antibody is highly efficient and specific, showing a clear target band with no nonspecific bands. I will definitely continue using BosterBio products and will recommend them to my classmates and colleagues.

IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective.

Excellent, submitted by on
SKU M00101-1
Application Western Blot
Sample Mouse pancreatic acinar cells
Sample Processing Description After centrifugation, collect the supernatant. Take a small portion for protein quantification using the BCA assay. Mix the remaining protein solution with an equal volume of loading buffer and denature in a 100°C water bath for 5 mins.
Primary Incubation The membrane was incubated with the IL-6 and IL-1β primary antibodies (1:1000) overnight at 4 °C.
Secondary Antibody HRP-conjugated Goat Anti-Rabbit IgG Secondary Antibody
Secondary Incubation Incubate at room temperature for 1 hour
Other Reagents used Protein-Free Blocking Buffer
Detection Signal was developed using ECL substrate on a ChemiDoc MP system.
Results Summary IL-6 and IL-1β levels in the pancreas are key for assessing the anti-inflammatory effect of this nanomedicine. Antibodies from two previous suppliers showed poor specificity, while BosterBio antibodies proved highly specific, potent, and cost-effective. In our subsequent experiments, our group continued to use BosterBio products, which proved to be highly reliable and provided solid support for the publication of several articles.