| SKU | P30433 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Spinal |
| Sample Processing Description | Tissue samples were directly lysed in RIPA lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Then, 20 μl of each cell protein sample was loaded into each lane of the SDS-PAGE gel. |
| Primary Antibody | Phospho GRF-1 (Tyr1105) Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Sangon Biotech |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |