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| SKU | DZ41119 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish retinal cryo-section |
| Sample Processing Description | Embryos fixed in 4% PFA for 4h. Embryos washed in PBST and 30% then 50% sucrose. Embedded in OCT and cryo-sectioned at 20nm |
| Primary Antibody | Zebrafish Tfap2a Antibody |
| Primary Incubation | 1:100, overnight at 4 ℃ |
| Detection | Used a Nikon C2+ confocal microscope |
| Results Summary | Was able to detect tfap2a expression in the retinal ganglion cells and anterior segment at 3dpf. |
| SKU | P30433 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Spinal |
| Sample Processing Description | Tissue samples were directly lysed in RIPA lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Then, 20 μl of each cell protein sample was loaded into each lane of the SDS-PAGE gel. |
| Primary Antibody | Phospho GRF-1 (Tyr1105) Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Sangon Biotech |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
| SKU | DZ01481 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:200 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in blocking solution. |
| SKU | DZ4103 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 488 goat anti-rabbit and was used in a 1:800 dilution. |
| SKU | DZ41032 |
|---|---|
| Application | Immunofluorescence |
| Sample | wildtype and KO mutant zebrafish retina |
| Primary Incubation | 1:100 |
| Blocking Agent | 10% normal goat serum and 2% bovine serum albumin in PBS |
| Secondary Antibody | Alexa Fluor 568 goat anti-rabbit was used in a 1:800 dilution. |
Source: Biocompare.com
| SKU | DZ41383 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish WKM, IPEX and FFPE tissue sections |
| Primary Incubation | Overnight |
| Blocking Agent | 3% goat serum in PBS |
| Secondary Incubation | 1 hour |
| Tertiary Incubation | None |
| Detection | Fluorescence microscopy |
| DOI or PMID # | https://doi.org/10.1101/2024.04.29.591640 |
| Results Summary | Antibody detects eosinophils and stains eosinophil granula. It is highly specific and can be used in dilutions such as 1:1000 for IF. |
"We ordered this antibody as a custom antibody from Boster Bio to stain for zebrafish eosinophils in FFPE tissue sections and fixed cells. It works wonderfully: very bright, with no relevant background or off-target staining, and highly reproducible."
| SKU | DZ01398-1 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish heart |
| Detection | Confocal Microscopy |
"Mef2a was detected in a cryosection of zebrafish heart using the rabbit anti-Mef2a antibody (cat# DZ01398-1, 1:200, 4 degrees overnight). AlexaFluor546 Conjugated Goat Anti-Rabbit IgG was used as a secondary antibody at 1:200 dilution and incubated for 1.5 h at room temperature."
Source: Biocompare.com
| SKU | DZ07988 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos, 5 days old |
| Detection | Confocal Microscopy |
"The antibody clearly labelled the outer segments of photoreceptor cells. The antibody is clean and precise. A zebrafish specific antibody for cdhr1a that WORKS!"
Source: Biocompare.com
| SKU | DZ02095 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 5dpf |
| Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"
Source: Biocompare.com
| SKU | DZ12892 |
|---|---|
| Application | Immunofluorescence |
| Sample | Zebrafish embryos 24hpf |
| Detection | Confocal Microscopy |
"Zebrafish-specific antibody. Used in whole mount IF on 24hpf zebrafish embryos. The signal is not precisely clean, but we do observe nlz2 expression in the ventral region of the retina as expected from in situ hybridization results. This antibody shows some promising results in whole mount applications using 24hpf embryos. Applications with cryosections may be more precise."