| SKU | P30433 |
|---|---|
| Application | Western Blot |
| Sample | Mouse Spinal |
| Sample Processing Description | Tissue samples were directly lysed in RIPA lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98ยฐC. Then, 20 ฮผl of each cell protein sample was loaded into each lane of the SDS-PAGE gel. |
| Primary Antibody | Phospho GRF-1 (Tyr1105) Antibody |
| Primary Incubation | 1:1000, overnight at 4 โ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Sangon Biotech |
| Results Summary | I will purchase Boster products again and recommend them to my classmates and colleagues. |
