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In the IF experiment using Anti-IBA-1 antibody (Cat# M01394-4), the antibody clearly and specifically labeled microglial cells in mouse cerebral infarction tissue, showing distinct staining and well-defined cellular morphology.

Excellent, submitted by Yu Lei, School of Life Sciences, Wuhan University on 2026-02-26

SKU	M01394-4
Application	Immunofluorescence
Sample	mouse brain tissue
Sample Processing Description	Mouse cerebral infarction model; brain tissues were collected, fixed in formaldehyde for 48 hours, and then sagittally paraffin-embedded.
Other Reagents	Goat serum, DAPI Staining Solution, Antifade fluorescence mounting medium.
Primary Antibody	Iba1 Rabbit Monoclonal Antibody
Primary Incubation	1:100, overnight at 4 ℃
Secondary Antibody	Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro594 Conjugated (BA1142, Boster)
Secondary Incubation	45 min at 37℃
Detection	Imaging system:Leica DMi3000
Results Summary	IBA-1 is a well-established marker of microglia in the nervous system. Microglia are the primary immune effector cells in the central nervous system and respond rapidly to CNS injury by proliferating, upregulating or re-expressing MHC antigens, migrating, and adopting a phagocyte-like morphology. In this study, IBA-1 was used to label microglia in cerebral infarction samples, showing clear staining and accurate cellular morphology.

This antibody exhibits high specificity and clear bands, and the resulting experimental data are reliable, providing important support for elucidating the molecular mechanisms underlying the role of PTN in this study.

Excellent, submitted by Zhixiong Liu, Xiamen University on 2025-10-30

SKU	P30433
Application	Western Blot
Sample	Mouse Spinal
Sample Processing Description	Tissue samples were directly lysed in RIPA lysis buffer, mixed with loading buffer at the appropriate ratio, and denatured by heating at 98°C. Then, 20 μl of each cell protein sample was loaded into each lane of the SDS-PAGE gel.
Primary Antibody	Phospho GRF-1 (Tyr1105) Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Blocking Agent	5% non-fat milk
Secondary Antibody	HRP-conjugated anti-rabbit IgG
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Substrate: ECL substrate, Imaging system: Sangon Biotech
Results Summary	I will purchase Boster products again and recommend them to my classmates and colleagues.