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Product Info Summary
|Description:||Transient overexpression lysate of sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant 1|
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alpha Sarcoglycan (SGCA) (NM_000023) Human Over-expression Lysate
Transient overexpression lysate of sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) (SGCA), transcript variant 1
Storage & Handling
Cite This Product
alpha Sarcoglycan (SGCA) (NM_000023) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006442)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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alpha Sarcoglycan (SGCA) Human Over-expression Lysates NM_000023.
Western blot validation of overexpression lysate (LS006442) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SGCA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SGCA (Source: Uniprot.org, NCBI)
sarcoglycan alpha/epsilon family
50-DAG; Adhalin; ADL; alphaSarcoglycan; alpha-Sarcoglycan; DAG2; DMDA2; DMDA250kD DAG; Dystroglycan-2; LGMD2D; LGMD2Dalpha (50kD dystrophin-associated glycoprotein); sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); SCARMD1; SGCA SGCA 50DAG, ADL, DAG2, DMDA2, LGMD2D, LGMDR3, SCARMD1, adhalin sarcoglycan alpha alpha-sarcoglycan|50 kDa dystrophin-associated glycoprotein|50kD DAG|alpha-SG|dystroglycan-2|limb girdle muscular dystrophy 2D|sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SGCA, check out the SGCA Infographic
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In this infographic, you will see the following information for SGCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For alpha Sarcoglycan (SGCA) (NM_000023) Human Over-expression Lysate (LS006442)
No publications found for LS006442
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