APP antibody (PB9091) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 15 Publications
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Anti-beta Amyloid/APP Antibody Picoband®

APP antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitIF, IHC, ICC, WB

SKU: PB9091

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

2 in stock. Ships Next Business Day.

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Tested Applications

APP antibody was positively detected by WB in:

human Hela whole cell, human U-87MG whole cell, human T-47D whole cell, human A549 whole cell, human U2OS whole cell, rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

APP antibody was positively detected by IHC in:

human glioma tissue, mouse brain tissue, human renal cancer tissue, human tonsil tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

APP antibody was positively detected by ICC/IF in:

A431 cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat, Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-beta Amyloid/APP Antibody Picoband®
View all APP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human APP, different from the related mouse and rat sequences by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated Molecular Weight

86.9 kDa

Background of APP
beta Amyloid, also called Abeta or Abeta, denotes peptides of 36–43 amino acidsthat are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. It is mapped to 19q13.12. Several potential activities have been discovered for beta Amyloid, including activation of kinase enzymes, functioning as atranscription factor, and anti-microbial activity (potentially associated with beta Amyloid's pro-inflammatoryactivity). Moreover, monomeric beta Amyloid is indicated to protect neurons by quenching metal-inducible oxygen radical generation and thereby inhibiting neurotoxicity.
Cite This Product
Anti-beta Amyloid/APP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9091)

Protocols

Western Blot protocol for APP antibody
Immunohistochemistry (IHC) protocol for APP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for APP antibody

Publications

Application:
Species:
Prolonged sevoflurane exposure induces exaggerated and persistent cognitive decline in old rats with pre-existing impairment by increasing hippocampal mitochondrial dysfunction and apoptosis
Published: 2026-07-31
Journal: NEUROTOXICOLOGY
Application: IHC
Impact Factor: 3.80
Species: Rat
PMID: 42537810
Impact of hyaluronic acid-modified hafnium metalorganic frameworks containing rhynchophylline on Alzheimer’s disease
Published: 2025-03-18
Journal: Open Life Sciences
Application: IF
Impact Factor: 1.70
Species: Mouse
PMID: 40109770
Developing diagnostic biomarkers for Alzheimer's disease based on histone lactylation-related gene
Published: 2024-09-11
Journal: Heliyon
Application: IHC,WB
Impact Factor: 3.40
Species: Mouse
PMID: 39315143
Macromolecular nanoparticles to attenuate both reactive oxygen species and inflammatory damage for treating Alzheimer's disease
Published: 2022-11-29
Journal: Bioengineering & Translational Medicine
Application: IF
Impact Factor: 10.68
Species: Mouse
PMID: 37206236
Effects of alcohol intake on cognitive function and β-amyloid protein in APP/PS1 transgenic mice
Published: 2021-03-16
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: IHC
Impact Factor: 6.03
Species: Mouse
PMID: 33737111
Systematic and experimental investigations of the anti-colorectal cancer mediated by genistein
Published: 2020-09-20
Journal: BIOFACTORS
Application: IF
Impact Factor: 4.73
Species: Human,Mouse
PMID: 32951326
Meloxicam Improves Cognitive Impairment of Diabetic Rats through COX2-PGE2-EPs-cAMP/pPKA Pathway
Published: 2018-08-15
Journal: MOLECULAR PHARMACEUTICS
Application: WB
Impact Factor: 4.56
Species: Rat
PMID: 30109938
α‑lipoic acid can greatly alleviate the toxic effect of AGES on SH‑SY5Y cells
Published: 2018-02-08
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: ICC
Impact Factor: 2.78
Species: Human
PMID: 29436603
Nicorandil inhibits oxidative stress and amyloid-β precursor protein processing in SH-SY5Y cells overexpressing APPsw
Published: 2015-02-15
Journal: International Journal of Clinical and Experimental Medicine
Application: WB
Impact Factor: 1.28
Species: Human
PMID: 25932125
Combined administration of D -galactose and aluminium induces Alzheimerlike lesions in brain
Published: 2011-06-04
Journal: Neuroscience Bulletin
Application: IHC
Impact Factor: 0.00
Species: Mouse
PMID: 21614097
Icariin inhibits beta-amyloid peptide segment 25–35 induced expression of β-secretase in rat hippocampus
Published: 2009-09-24
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 2.79
Species: Rat
PMID: 19782061
Effect of chronic intermittent hypoxia on the expression of Nip3, cell apoptosis, β-amyloid protein deposit in mice brain cortex
Published: 2009-01-01
Journal: CHINESE MEDICAL JOURNAL
Application: IHC
Impact Factor: 0.86
Species: Mouse
Protection of Mouse Brain from Aluminum-induced Damage by Caffeic Acid
Published: 2008-03-14
Journal: CNS Neuroscience & Therapeutics
Application: IHC
Impact Factor: 0.00
Species: Mouse
PMID: 18482095
Protective effects of meloxicam on aluminum overload-induced cerebral damage in mice
Published: 2006-07-25
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 2.48
Species: Mouse
PMID: 16928369
Protective effects of Ginkgo biloba leaf extract on aluminum-induced brain dysfunction in rats
Published: 2005-02-25
Journal: LIFE SCIENCES
Application: IHC
Impact Factor: 2.16
Species: Rabbit
PMID: 15862599

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Product Promise

We promise Anti-beta Amyloid/APP Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more

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PB9091
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Sep 30, 2026