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- Table of Contents
ARG2 antibody (A02244) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of Jurkat cells using anti-ARG2 antibody (A02244).
Overlay histogram showing Jurkat cells stained with A02244 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARG2 Antibody (A02244, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Jurkat cells stained with A02244 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARG2 Antibody (A02244, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing a Western blot for ARG2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ARG2 Western Blot GuideBundle Save
Anti-ARG2 Antibody Picoband®
ARG2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ARG2 antibody was positively detected by WB in:
human HepG2 cells, human K562 cells, human 293T cells, human Caco-2 cells, rat kidney tissue, rat colon tissue, mouse kidney tissue, mouse colon tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ARG2 antibody was positively detected by IHC in:
human lung cancer tissue, human prostate cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ARG2 antibody was positively detected by ICC/IF in:
U2OS cell
ARG2 antibody was positively detected by FC in:
Jurkat cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ARG2 Antibody Picoband®
View all ARG2 Antibodies
View all ARG2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ARG2 recombinant protein (Position: M1-I354).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
38.6 kDa
Background of ARG2
ARG2 (arginase, type II) encodes a 355-amino acid polypeptide.Using Northern blotting and RT-PCR,Vockley et al. (1996)found thatARG2is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney.By PCR analysis of somatic cell hybrid panels, fluorescence in situ hybridization, and radiation hybrid analysis,theARG2 gene is mapped to 14q24.1-q24.3. ARG2may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al. (1996)showed thatARG2mRNA and nitric oxide synthase (NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location ofARG2and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference thatARG2is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle.
Cite This Product
Anti-ARG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02244)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
94%
High Homology
Cow
92%
High Homology
Sheep
92%
High Homology
Rabbit
88%
Moderate Homology
Mouse
85%
Moderate Homology
Rat
84%
Moderate Homology
Zebrafish
61%
Low homology
Drosophila
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ARG2 antibody
Immunohistochemistry (IHC) protocol for ARG2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ARG2 antibody
Flow Cytometry (FC) protocol for ARG2 antibody
Publications
Application:
Species:
Kaempferol Protects Pulmonary Vascular Endothelial Function in Rats with High Altitude Pulmonary Hypertension by Regulating RAS System and AMPK/Arg2/eNOS Signaling Pathway
Published: 2024-08-22
Journal: Natural Product Communications
Application: WB
Impact Factor: 1.50
Species: Rat
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Product Promise
We promise Anti-ARG2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02244
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026