Anti-ASCL1/Mash1 Antibody

Product Name

Anti-ASCL1/Mash1 Antibody

View all ASCL1/Mash1 Antibodies

SKU/Catalog Number

A03023

Size

100μl

Form

Liquid

Description

Boster Bio Anti-ASCL1/Mash1 Antibody catalog # A03023. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ASCL1/Mash1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03023)

Host

Rabbit

Contents

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide from human protein at AA range: 190-236.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03023 is reactive to ASCL1 in Human, Mouse, Rat

Applications

A03023 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

25.454kDa

Background of ASCL1/Mash1

Anti-Cul2 antibody can be used in western blotting, ELISA and IP. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC-P 1:50-200
ELISA 1:10000-20000

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.

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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.

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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.

Gene/Protein Information For ASCL1 (Source: Uniprot.org, NCBI)

Alternative Names

achaete-scute complex (Drosophila) homolog-like 1; achaete-scute complex homolog 1 (Drosophila); achaete-scute complex-like 1 (Drosophila); achaete-scute homolog 1; ASCL1; ASH1; ASH-1; ASH1MASH1; BHLHA46; bHLHa46achaete-scute complex-like 1; Class A basic helix-loop-helix protein 46; HASH1; HASH1achaete scute protein; Mash1 ASCL1 ASH1, HASH1, MASH1, bHLHa46 achaete-scute family bHLH transcription factor 1 achaete-scute homolog 1|ASH-1|achaete scute protein|achaete-scute complex homolog 1|achaete-scute complex-like 1|class A basic helix-loop-helix protein 46

For more info on ASCL1, check out the ASCL1 Infographic

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In this infographic, you will see the following information for ASCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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