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Product Info Summary
| SKU: | AZF1RDC0 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish ATG5 Antibody Picoband®
SKU/Catalog Number
AZF1RDC0
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish ATG5 Antibody Picoband® catalog # AZF1RDC0. Tested in IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Immunohistochemistry applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20 ℃ for one year from date of receipt. After reconstitution, at 4 ℃for one month. It can also be aliquotted and stored frozen at -20 ℃for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish ATG5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1RDC0)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish ATG5 recombinant protein (Position: S118-L155).
Reactive Species
AZF1RDC0 is reactive to ATG5 in Zebrafish
Background of ATG5
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZF1RDC0 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Positive Control
IHC: zebrafish cerebellum tissue
Validation Images & Assay Conditions
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IHC analysis of Zebrafish ATG5 using anti-Zebrafish ATG5 antibody (AZF1RDC0).
Zebrafish ATG5 was detected in paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish ATG5 Antibody (AZF1RDC0) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish ATG5 Antibody Picoband® (AZF1RDC0)
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