HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Cited in 10 publication(s).

Product Info Summary

SKU: BA1054
Size: 0.5ml
Reactive Species: Rabbit
Host: Goat
Application: ELISA, WB

Product Overview

Product Name HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Synonyms HRP-conjugated goat anti-rabbit IgG
Description Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Goat
Target Species Rabbit
Antibody Class IgG
Clonality Polyclonal
Immunogen Rabbit IgG (whole molecular)
Purification The antibody was purified from antisera by immunoaffinity chromatography.
Specificity This HRP conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.
Form Supplied Concentrated, Liquid
Formulation 0.5 mg of HRP conjugated specific antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1mg/ml
Application ELISA*, WB*
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western Blotting: 0.1-0.2μg/ml (ECL detection)
Western Blotting: 0.7-3.3μg/ml (DAB detection)
Direct ELISA: 0.05-0.5μg/ml (TMB detection)

Validation Images & Assay Conditions

BA1054 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Upregulation of ATG4A promotes osteosarcoma cell epithelial-to-mesenchymal transition through the Notch signaling pathway

Exosomes secreted by HUVECs attenuate hypoxia/reoxygenation-induced apoptosis in neural cells by suppressing miR-21-3p

MiR-514a-3p inhibits cell proliferation and epithelial-mesenchymal transition by targeting EGFR in clear cell renal cell carcinoma

Hypoxia promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells via inducing Twist1 expression.

Nogo-A antibody treatment enhances neuron recovery after sciatic nerve transection in rats.

Calpain 1 regulates TGF-β1-induced epithelial-mesenchymal transition in human lung epithelial cells via PI3K/Akt signaling pathway

ERK/CANP rapid signaling mediates 17β-estradiol-induced proliferation of human breast cancer cell line MCF-7 cells

Protective mechanism of kaempferol against Aβ25-35-mediated apoptosis of pheochromocytoma (PC-12) cells through the ER/ERK/MAPK signalling pathway

Inhibition of proliferation, migration and invasion of human non-small cell lung cancer cell line A549 by phlomisoside F from Phlomis younghusbandii Mukerjee

Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model

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2 Customer Q&As for HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

Question

Would either BA1054 secondary antibody work for M00139 for flow cytometry?

Verified customer

Asked: 2019-11-25

Answer

We don't recommend the Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate (BA1054) for M00139 for flow cytometry.

Boster Scientific Support

Answered: 2019-11-25

Question

What type of concentration and the state of the preservatives was used for the BA1054 secondary antibody? Is it liquid or lyophilized?

Verified customer

Asked: 2019-10-29

Answer

The Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate BA1054 is concentrated, Liquid with a concentration of 1 mg/ml. The contents are as followed: 0.5 mg of peroxidase conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.

Boster Scientific Support

Answered: 2019-10-29

Size

Total: $95

SKU:BA1054

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