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SKU BA1054
Pack size 0.5 ml
Applications Western blot

Product Overview

Product Name Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate
Synonyms HRP-conjugated goat anti-rabbit IgG
Description Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dot Blot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex.
Reagent Type Secondary antibody, reporter enzyme labeled
Label HRP (Horseradish Peroxidase)
Host Goat
Target Species Rabbit
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule rabbit IgG
Purification Immunoaffinity Chromatography
Specificity Rabbit IgG specific; No cross-reactivity with related species
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg HRP-conjugated secondary antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA*, WB*, Dot blot
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage 4C for 1 year

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed WB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer
Compatibility with Reagents Incompatible with sodium azide and metals
incompatible with high phosphate concentrations

Main Advantages

Specific High signal-to-noise ratio
Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC
Fast Generates strong signals in a relatively short time span
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates;
Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes


Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Images

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Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate
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Toll-like receptor 4 implicated in acute lung injury induced by paraquat poisoning in mice
17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway
Glimepiride Promotes Osteogenic Differentiation in Rat Osteoblasts via the PI3K/Akt/eNOS Pathway in a High Glucose Microenvironment
Ginsenoside Rb1 Prevents H2O2-Induced HUVEC Senescence by Stimulating Sirtuin-1 Pathway
Overexpression of Rcan1-1L Inhibits Hypoxia-Induced Cell Apoptosis through Induction of Mitophagy
Evaluating the Role of PTH in Promotion of Chondrosarcoma Cell Proliferation and Invasion by Inhibiting Primary Cilia Expression
Tolerance of neurite outgrowth to Rho kinase inhibitors decreased by cyclooxygenase-2 inhibitor
Single Prolonged Stress induces ATF6 alpha-dependent Endoplasmic reticulum stress and the apoptotic process in medial Frontal Cortex neurons
Stanniocalcin 2 expression predicts poor prognosis of hepatocellular carcinoma
Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells
IL-17 regulates the expressions of RANKL and OPG in human periodontal ligament cells via TRAF6/TBK1-JNK/NF-?B pathways.
Expression and function of Delta-like ligand 4 in a rat model of retinopathy of prematurity
PD-L1 is increased in the spinal cord and infiltrating lymphocytes in experimental allergic encephalomyelitis
ClC-3 chloride channel in hippocampal neuronal apoptosis
Chloride channel blocker 4,4-diisothiocyanatostilbene-2,2?-disulfonic acid inhibits nitric oxide-induced apoptosis in cultured rat hippocampal neurons
Changes of hypoxia-inducible factor-1 signaling and the effect of cilostazol in chronic cerebral ischemia
Radix Achyranthis Bidentatae improves learning and memory capabilities in ovariectomized rats
Effect of minocycline on cerebral ischemia-reperfusion injury
Effect of Vitamin E Supplementation on Intestinal Barrier Function in Rats Exposed to High Altitude Hypoxia Environment
Zinc Supplementation Protects against Cadmium Accumulation and Cytotoxicity in Madin-Darby Bovine Kidney Cells
Roles of TLR3 and RIG-I in Mediating the Inflammatory Response in Mouse Microglia following Japanese Encephalitis Virus Infection
Oxygen-Carbon Nanotubes as a Chemotherapy Sensitizer for Paclitaxel in Breast Cancer Treatment
Apelin-13 Decreases Lipid Storage in Hypertrophic Adipocytes?In Vitro?Through the Upregulation of AQP7 Expression by the PI3K Signaling Pathway
Swainsonine Induces Apoptosis through Mitochondrial Pathway and Caspase Activation in Goat Trophoblasts
Guanxinkang Decoction Exerts Its Antiatherosclerotic Effect Partly through Inhibiting the Endoplasmic Reticulum Stress
FHL1C induces apoptosis in notch1-dependent T-ALL cells through an interaction with RBP-J
Urantide improves atherosclerosis by controlling C-reactive protein, monocyte chemotactic protein-1 and transforming growth factor-? expression in rats
Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor ?B pathway
Powerful inner/outer controlled multi-target magnetic nanoparticle drug carrier prepared by liquid photo-immobilization
Mest Attenuates CCl4-Induced Liver Fibrosis in Rats by Inhibiting the Wnt/?-Catenin Signaling Pathway
Periodontal Ligament Stem Cells Modulate Root Resorption of Human Primary Teeth via Runx2 Regulating RANKL/OPG System
Protective effect of glutamine on intestinal injury and bacterial community in rats exposed to hypobaric hypoxia environment
The?Streptococcus suis?transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid
The Low Chamber Pancreatic Cancer Cells Had Stem-Like Characteristics in Modified Transwell System: Is It a Novel Method to Identify and Enrich Cancer Stem-Like Cells?
Immunization with H7-HCP-Tir-Intimin Significantly Reduces Colonization and Shedding of?Escherichia coli?O157:H7 in Goats
Comparative study of the immunogenicity in mice and monkeys of an inactivated CA16 vaccine made from a human diploid cell line
Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis
ERK/CANP rapid signaling mediates 17?-estradiol-induced proliferation of human breast cancer cell line MCF-7 cells
Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells
N-n-Butyl Haloperidol Iodide Ameliorates Cardiomyocytes Hypoxia/Reoxygenation Injury by Extracellular Calcium-Dependent and -Independent Mechanisms
Dickkopf-1 Is Oncogenic and Involved in Invasive Growth in Non Small Cell Lung Cancer
Role of the Hypoxia-inducible factor-1 alpha induced autophagy in the conversion of non-stem pancreatic cancer cells into CD133+ pancreatic cancer stem-like cells
LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn?s disease
Inhibition of choriocarcinoma by Fe3O4-dextran-anti-?-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase
Hypoxia/oxidative stress alters the pharmacokinetics of CPU86017-RS through mitochondrial dysfunction and NADPH oxidase activation
Heat Shock Protein 70 Is Associated with Replicase Complex of Japanese Encephalitis Virus and Positively Regulates Viral Genome Replication
Electrospun Poly(L-lactide)/Poly(?-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells
The Time Course Effects of Electroacupuncture on Promoting Skeletal Muscle Regeneration and Inhibiting Excessive Fibrosis after Contusion in Rabbits
Estrogen deficiency reduces the dentinogenic capacity of rat lower incisors
Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway
Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses
The expression and distribution of aquaporin 3 in mouse embryos before and after vitrification
AngII-induced glomerular mesangial cell proliferation inhibited by losartan via changes in intracellular calcium ion concentration
Serum GP73, a Marker for Evaluating Progression in Patients with Chronic HBV Infections
Transient overexpression of TGFBR3 induces apoptosis in human nasopharyngeal carcinoma CNE-2Z cells
Caveolin-1 Regulates Rac1 Activation and Rat Pulmonary Microvascular Endothelial Hyperpermeability Induced by TNF-?
Cellular Mechanism Underlying Formaldehyde-Stimulated Cl??Secretion in Rat Airway Epithelium
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and?Staphylococcus aureus
Pro-survival effect of Dock180 overexpression on rat-derived H9C2 cardiomyocytes
Excessive Astrocyte-Derived Neurotrophin-3 Contributes to the Abnormal Neuronal Dendritic Development in a Mouse Model of Fragile X Syndrome
Elevated expression of C3G protein in the peri-infarct myocardium of rats
Cloning of the Quail PIWI Gene and Characterization of PIWI Binding to Small RNAs
Antibody Fragments Directed against Different Portions of the Human Neural Cell Adhesion Molecule L1 Act as Inhibitors or Activators of L1 Function
Molecular Adjuvant Ag85A Enhances Protection against Influenza A Virus in Mice Following DNA Vaccination
Temporal and Spatial Regulation of Ezrin-Radixin-Moesin-Binding Phosphoprotein-50-kDa (EBP50) during Embryo Implantation in Mouse Uterus
Adenoviral 15-lipoxygenase-1 gene transfer inhibits hypoxia-induced proliferation of retinal microvascular endothelial cells in vitro
In vitro and in vivo studies of surface-structured implants for bone formation
Special AT-rich sequence binding protein 1 regulates the multidrug resistance and invasion of human gastric cancer cells
Down-Regulation of GEP100 Causes Increase in E-Cadherin Levels and Inhibits Pancreatic Cancer Cell Invasion
Down-Regulation of AP-4 Inhibits Proliferation, Induces Cell Cycle Arrest and Promotes Apoptosis in Human Gastric Cancer Cells
Expression of neuroglobin in ocular hypertension induced acute hypoxic-ischemic retinal injury in rats
The Extra Domain A of Fibronectin Increases VEGF-C Expression in Colorectal Carcinoma Involving the PI3K/AKT Signaling Pathway
CPU86017-RS attenuate hypoxia-induced testicular dysfunction in mice by normalizing androgen biosynthesis genes and pro-inflammatory cytokines
Serine 129 Phosphorylation of??-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies
Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of
Berberine Improves Glucose Metabolism in Diabetic Rats by Inhibition of Hepatic Gluconeogenesis
Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks
Estrogen Receptor-Related Receptor ? Mediates Up-Regulation of Aromatase Expression by Prostaglandin E2 in Prostate Stromal Cells
Identification of 20-Hydroxyecdysone Late-Response Genes in the Chitin Biosynthesis Pathway
Lowered HGK expression inhibits cell invasion and adhesion in hepatocellular carcinoma cell line HepG2
Acute Stress and Chronic Stress Change Brain-Derived Neurotrophic Factor (BDNF) and Tyrosine Kinase-Coupled Receptor (TrkB) Expression in Both Young and Aged Rat Hippocampus
Nucleolin/C23 is a negative regulator of hydrogen peroxide-induced apoptosis in HUVECs
Different Functions of the Insect Soluble and Membrane-Bound Trehalase Genes in Chitin Biosynthesis Revealed by RNA Interference
Investigating the Role of P311 in the Hypertrophic Scar
Expression of indoleamine 2,3-dioxygenase in nasopharyngeal carcinoma impairs the cytolytic function of peripheral blood lymphocytes
Detection of B. anthracis Spores and Vegetative Cells with the Same Monoclonal Antibodies
Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation
LFA-1 and CD2 Synergize for the Erk1/2 Activation in the Natural Killer (NK) Cell Immunological Synapse
Over-expressed and truncated midkines promote proliferation of BGC823 cells
Upregulation of the constitutively expressed HSC70 by KLF4
Release of heat shock protein 70 and the effects of extracellular heat shock protein 70 on the production of IL-10 in fibroblast-like synoviocytes
Expression of Mipu1 in Response to Myocardial Infarction in Rats
Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
Y4lO of Rhizobium sp. Strain NGR234 Is a Symbiotic Determinant Required for Symbiosome Differentiation
Symbiosis-Promoting and Deleterious Effects of NopT, a Novel Type 3 Effector of Rhizobium sp. Strain NGR234
Advanced Glycation End-Products Induce Connective Tissue Growth Factor-Mediated Renal Fibrosis Predominantly through Transforming Growth Factor ?-Independent Pathway
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Customer Q&As

Q: What species have been tested for the cross-reactivity of BA1054 Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate ?
A: Mouse and Bovine has been tested. It showed no cross-reactivity with mouse/bovine IgG.
Q: Is this product suitable for IHC? Keyword: application, Immunohistochemistry
A: Please beware that this product is not suitable for IHC. Small package of secondaries (0.5 ml) can do 15-75 slides and 1ml can do 30-150 slides. The difference is in the way customer uses the reagent.