Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374

c-Fos antibody

Boster Bio Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 (Catalog # M00297-5). Tested in IHC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M00297-5
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374

View all c-Fos Antibodies

SKU/Catalog Number

M00297-5

Size

100uL

Form

Liquid

Description

Boster Bio Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 (Catalog # M00297-5). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297-5)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM374

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to N-terminus of human Proto-oncogene c-Fos

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human Proto-oncogene c-Fos. It may also react to mouse and rat c-Fos, as predicted by immunogen homology.

Reactive Species

M00297-5 is reactive to FOS in Human

Applications

M00297-5 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

40.695kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:5000 dilution.

Validation Images & Assay Conditions

Gene/Protein Information For FOS (Source: Uniprot.org, NCBI)

Gene Name

FOS

Full Name

Proto-oncogene c-Fos

Weight

40.695kDa

Superfamily

bZIP family

Alternative Names

activator protein 1; AP-1; cellular oncogene c-fos; Cellular oncogene fos; cFos; c-Fos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FOS; G0/G1 switch regulatory protein 7; G0S7; proto-oncogene c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FOS, check out the FOS Infographic

FOS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00297-5 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil

miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor

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16 Customer Q&As for Anti-c-Fos Rabbit Monoclonal Antibody, Clone#RM374

Question

Is a blocking peptide available for product anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 (M00297-5)?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

We do provide the blocking peptide for product anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 (M00297-5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-31

Question

Do you have a BSA free version of anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 available?

T. Moore

Verified customer

Asked: 2020-02-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-25

Question

We are interested in using your anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 for response to muscle stretch studies. Has this antibody been tested with western blotting on thyroid cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

Thank you for your inquiry. This M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 is validated on thyroid cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5. Let me know if you need anything else.

P. Li

Verified customer

Asked: 2019-07-03

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-03

Question

Our team were satisfied with the WB result of your anti-c-Fos Rabbit Monoclonal antibody, Clone

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

Based on literature, pancreas does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-05

Question

I see that the anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-21

Question

Does M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

As indicated on the product datasheet, M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-16

Question

you antibody to test anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 on human pancreas for research purposes, then I may be interested in using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-04-09

Answer

The products we sell, including anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-04-09

Question

We have been able to see staining in human leukemic t-cell. Do you have any suggestions? Is anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 supposed to stain leukemic t-cell positively?

K. Bhatt

Verified customer

Asked: 2019-01-21

Answer

According to literature leukemic t-cell does express FOS. According to Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-01-21

Question

Here is the WB image, lot number and protocol we used for pancreas using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-06-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-27

Question

Will anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 work for WB with pancreas?

D. Miller

Verified customer

Asked: 2018-02-26

Answer

According to the expression profile of pancreas, FOS is highly expressed in pancreas. So, it is likely that anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 will work for WB with pancreas.

Boster Scientific Support

Answered: 2018-02-26

Question

Is this M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 reactive to the isotypes of FOS?

Verified Customer

Verified customer

Asked: 2018-02-07

Answer

The immunogen of M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 is A peptide corresponding to N-terminus of human Proto-oncogene c-Fos. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-07

Question

We are currently using anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 M00297-5 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

C. Taylor

Verified customer

Asked: 2016-08-17

Answer

The anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 (M00297-5) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-17

Question

I was wanting to use your anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 for WB for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?

J. Baker

Verified customer

Asked: 2015-04-14

Answer

It shows on the product datasheet, M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-04-14

Question

I have a question about product M00297-5, anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Evans

Verified customer

Asked: 2014-10-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00297-5 anti-c-Fos Rabbit Monoclonal antibody, Clone#RM374, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-10-03

Question

We have tried in the past anti-c-Fos Rabbit Monoclonal antibody, Clone

Z. Johnson

Verified customer

Asked: 2014-02-07

Answer

I have checked the website and datasheets of our anti-c-Fos Rabbit Monoclonal antibody, Clone

Boster Scientific Support

Answered: 2014-02-07

$431
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Lead time for this item is typically 1-2 weeks

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M00297-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$431.00

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