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Product Info Summary
| SKU: | M03780-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272
SKU/Catalog Number
M03780-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272 (Catalog # M03780-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03780-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM272
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD20
Cross-reactivity
This antibody reacts to the cytoplasmic domain of human CD20.
Reactive Species
M03780-2 is reactive to MS4A1 in Human
Calculated molecular weight
33.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03780-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of Raji cell lysates using Anti-CD20 Rabbit Monoclonal Antibody (Clone RM272) at a 1:1000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections using Anti-CD20 Rabbit Monoclonal Antibody (Clone RM272) at a 1:1000 dilution.
Specific Publications For Anti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272 (M03780-2)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD20 MS4A1 Rabbit Monoclonal Antibody, Clone#RM272
Question
We are currently using anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 (M03780-2) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-28
Question
Is there a BSA free version of anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 available?
R. Walker
Verified customer
Asked: 2020-02-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
I have a question about product M03780-2, anti-CD20 Rabbit Monoclonal antibody, Clone#RM272. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-22
Question
Is this M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 reactive to the isotypes of MS4A1?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The immunogen of M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 is A peptide corresponding to the C-terminus of human CD20. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-05
Question
Is a blocking peptide available for product anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 (M03780-2)?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We do provide the blocking peptide for product anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 (M03780-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-16
Question
My boss were happy with the WB result of your anti-CD20 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
From what I have seen in literature, lymph does express MS4A1. Generally MS4A1 expresses in cell membrane. Regarding which tissues have MS4A1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 22615937
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-09-11
Question
We have observed staining in human t-cell. Any tips? Is anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2019-04-09
Answer
From literature t-cell does express MS4A1. From Uniprot.org, MS4A1 is expressed in blood, lymph, t-cell, b-cell, among other tissues. Regarding which tissues have MS4A1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 22615937
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-04-09
Question
We ordered your anti-CD20 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
I viewed the website and datasheets of our anti-CD20 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-07-17
Question
I see that the anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymph using anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-14
Question
See attached the WB image, lot number and protocol we used for lymph using anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2. Please let me know if you require anything else.
P. Brown
Verified customer
Asked: 2018-04-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-17
Question
Does M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
As indicated on the product datasheet, M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-02-07
Question
We want using your anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 for store-operated calcium entry studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.
M. Collins
Verified customer
Asked: 2018-01-22
Answer
I appreciate your inquiry. This M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 is tested on human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-01-22
Question
I was wanting to use your anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 for WB for human lymph on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymph identification?
S. Edwards
Verified customer
Asked: 2018-01-12
Answer
As indicated on the product datasheet, M03780-2 anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-12
Question
Does anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 work for WB with lymph?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
According to the expression profile of lymph, MS4A1 is highly expressed in lymph. So, it is likely that anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 will work for WB with lymph.
Boster Scientific Support
Answered: 2017-08-09
Question
We need to test anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 on human lymph for research purposes, then I may be interested in using anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Williams
Verified customer
Asked: 2014-03-27
Answer
The products we sell, including anti-CD20 Rabbit Monoclonal antibody, Clone#RM272 M03780-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-27
