Anti-CD3 epsilon (activation epitope) Purified Monoclonal Antibody

Product Name

Anti-CD3 epsilon (activation epitope) Purified Monoclonal Antibody

View all CD3E Antibodies

SKU/Catalog Number

M02675-4

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CD3 epsilon (activation epitope) Purified Monoclonal Antibody (Catalog# M02675-4). Tested in Flow Cytometry, IP, WB, IHC-F, ICC application(s). This antibody reacts with Mouse, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-CD3 epsilon (activation epitope) Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02675-4)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

APA1/1

Isotype

Mouse IgG1

Immunogen

Purified human CD3 proteins isolated from thymus. The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M02675-4 is reactive to CD3E in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

23.1 kDa

Background of CD3E

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02675-4 is guaranteed for Flow Cytometry, IP, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.
Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Validation Images & Assay Conditions

See full target information CD3E

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