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- Table of Contents
CD3E antibody (PB9093) performs well in IF, IHC, IHC-F, WB and shows reactivity with Chicken, Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IF analysis of CD3E and CD20 using anti-CD3E antibody (PB9093) and anti-CD20 antibody (M03780-5).
CD3E and CD20 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-CD3E antibody (PB9093) and mouse anti-CD20 antibody (M03780-5) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135), DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD3E and CD20 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-CD3E antibody (PB9093) and mouse anti-CD20 antibody (M03780-5) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135), DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CD3 epsilon/CD3E Antibody Picoband®
CD3E antibody
| Reactive Species | Host | Application |
|---|---|---|
| Chicken, Human, Mouse, Rat | Rabbit | IF, IHC, IHC-F, WB |
Tested Applications
CD3E antibody was positively detected by WB in:
human MOLT-4 whole cell, human Jurkat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CD3E antibody was positively detected by IHC in:
human tonsil tissue, mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CD3E antibody was positively detected by IHC-F in:
rat spleen tissue, mouse spleen tissue
CD3E antibody was positively detected by IF in:
human tonsil tissue, human tonsil tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat, Chicken
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Mouse, Rat
Immunocytochemistry
0.5-1μg/ml
Human
Immunofluorescence
2μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
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WB
IHC
IF
Human
Tested
Tested
Tested
Mouse
Tested
Recommend
Rat
Tested
Recommend
Product Information
Product Name
Anti-CD3 epsilon/CD3E Antibody Picoband®
View all CD3E Antibodies
View all CD3E Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
23.1 kDa
Background of CD3E
CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Cite This Product
Anti-CD3 epsilon/CD3E Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9093)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
82%
Moderate Homology
Rat
64%
Low homology
Cow
62%
Low homology
Mouse
61%
Low homology
Rabbit
61%
Low homology
Sheep
60%
Low homology
Dog
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CD3E antibody
Immunohistochemistry (IHC) protocol for CD3E antibody
Immunofluorescence (IF) protocol for CD3E antibody
Publications
Application:
Species:
Immunological Features of Peri-Implant Soft Tissue After Healing Abutment Dislodgement: A Comparative Human Study
Published: 2026-03-23
Journal: INTERNATIONAL DENTAL JOURNAL
Application: IHC
Impact Factor: 3.70
Species: Human
PMID: 41875836
The construction of recombinant DNA vaccine pVAX-ROP27-IL-2-IFN-γ and its immune enhancement effect against Eimeria tenella
Published: 2026-01-18
Journal: POULTRY SCIENCE
Application: IHC
Impact Factor: 4.20
Species: Chicken
PMID: 41579602
Resilience and Vulnerabilities of Tumor Cells under Purine Shortage Stress
Published: 2025-10-15
Journal: CLINICAL CANCER RESEARCH
Application: IHC
Impact Factor: 10.20
Species: Mouse
PMID: 40788171
IL-33 Contributes to the Pathological Changes of Hair Follicles in Psoriasis: A Potential Target for Psoriatic Alopecia
Published: 2023-03-12
Journal: Clinical Cosmetic and Investigational Dermatology
Application: IHC
Impact Factor: 2.30
Species: Mouse
PMID: 36936754
The involvement of Th1 cell differentiation in the anti-tumor effect of purified polysaccharide from Sanghuangporus vaninii in colorectal cancer via multi-omics analysis
Published: 2023-03-07
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: IHC
Impact Factor: 8.20
Species: Mouse
PMID: 36889619
CXCR4 knockout induces neuropathological changes in the MPTP-lesioned model of Parkinson's disease
Published: 2022-11-08
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Application: IF
Impact Factor: 6.63
Species: Mouse
PMID: 36368650
An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis
Published: 2022-07-29
Journal: Nature Communications
Application: IHC
Impact Factor: 17.69
Species: Mouse
PMID: 35906240
Combination of Chidamide-Mediated Epigenetic Modulation with Immunotherapy: Boosting Tumor Immunogenicity and Response to PD-1/PD-L1 Blockade
Published: 2021-08-16
Journal: ACS Applied Materials & Interfaces
Impact Factor: 9.23
Species: Mouse
PMID: 34433253
Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis
Published: 2020-11-21
Journal: Nano-Micro Letters
Application: IHC
Impact Factor: 12.26
Species: Mouse
PMID: 34138183
Effectiveness of immune therapy combined with chemotherapy on the immune function and recurrence rate of cervical cancer
Published: 2015-01-26
Journal: Experimental and Therapeutic Medicine
Application: FC
Impact Factor: 1.27
Species: Human
PMID: 25667679
Review
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1 Review For Anti-CD3 epsilon/CD3E Antibody Picoband®
Accurate localization and good imaging performance.
| SKU | PB9093 |
|---|---|
| Application | Immunofluorenscence |
| Sample | Mouse 4T1 cell xenograft tumor |
| Sample Processing Description | Fixed in 4% paraformaldehyde for 48 hours, followed by paraffin embedding and sectioning. |
| Primary Incubation | 1:200, overnight at 4 ℃ |
| Blocking Agent | Goat serum |
| Secondary Antibody | DyLight 550-conjugated goat anti-rabbit antibody. |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Laser confocal microscopy |
| Results Summary | The delivery time for antibodies is impressively fast — I usually receive the products within a week, which saves me a lot of valuable time. Both the pre-sales and after-sales support are efficient and reliable. Since my background is in chemistry, I often consulted Boster’s technical specialists about various details of biological experiments. They were always patient and thorough in their explanations, helping me avoid many detours during my experiments. Most importantly, these antibodies offer excellent value for money — they have strong binding performance, produce clear imaging results, and ensure a high experiment success rate, which has provided a solid foundation for my research. |
Yanhua Li，Shandong Normal University
Verified customer
Submitted 2025-10-16
Product Promise
We promise Anti-CD3 epsilon/CD3E Antibody Picoband® performs excellently in relevant applications for IF, IHC, IHC-F, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9093
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026