This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CHRNA5 antibody (A02359-2) performs well in Flow Cytometry, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
Filter:
Flow Cytometry analysis of U251 cells using anti-CHRNA5 antibody (A02359-2).
Overlay histogram showing U251 cells stained with A02359-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRNA5 Antibody (A02359-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U251 cells stained with A02359-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRNA5 Antibody (A02359-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband®
CHRNA5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, ICC, WB |
Tested Applications
CHRNA5 antibody was positively detected by WB in:
rat skeletal muscle tissue, HEPG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CHRNA5 antibody was positively detected by IHC in:
mouse intestine tissue, mouse cardiac muscle tissue, rat intestine tissue, rat cardiac muscle tissue, human prostatic cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CHRNA5 antibody was positively detected by FC in:
U251 cell, A549 cell, MCF-7 cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Immunocytochemistry
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Human
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband®
View all CHRNA5 Antibodies
View all CHRNA5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CHRNA5, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated Molecular Weight
53.1 kDa
Background of CHRNA5
Neuronal acetylcholine receptor subunit alpha-5 is a protein that in humans is encoded by the CHRNA5 gene. It is mapped to 15q25.1. The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).
Cite This Product
Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02359-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
97%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
91%
High Homology
Rat
88%
Moderate Homology
Mouse
85%
Moderate Homology
Zebrafish
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CHRNA5 antibody
Immunohistochemistry (IHC) protocol for CHRNA5 antibody
Flow Cytometry (FC) protocol for CHRNA5 antibody
Publications
Application:
Species:
Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA
Published: 2020-01-17
Journal: Marine Drugs
Application: FC
Impact Factor: 4.07
Species: Human
PMID: 31963558
Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth
Published: 2019-10-18
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 3.17
Species: Human
PMID: 31634461
Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets
Published: 2019-04-28
Journal: Marine Drugs
Application: WB
Impact Factor: 3.77
Species: Human
PMID: 31035425
Review
Have you used Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
$30 for a review with an image
Submit A ReviewProduct Promise
We promise Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02359-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 27, 2026