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Product Info Summary
| SKU: | A05293 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CTACK CCL27 Antibody
SKU/Catalog Number
A05293
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CTACK CCL27 Antibody catalog # A05293. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTACK CCL27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05293)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-C motif chemokine 27 at AA range: 51-100
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05293 is reactive to CCL27 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
12.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05293 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of mouse-kidney lysis using CCL27 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100
Specific Publications For Anti-CTACK CCL27 Antibody (A05293)
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Customer Q&As
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4 Customer Q&As for Anti-CTACK CCL27 Antibody
Question
We are currently using anti-CTACK antibody A05293 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
The anti-CTACK antibody (A05293) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-05
Question
Will anti-CTACK antibody A05293 work on horse WB with thymus?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Our lab technicians have not validated anti-CTACK antibody A05293 on horse. You can run a BLAST between horse and the immunogen sequence of anti-CTACK antibody A05293 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse thymus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-26
Question
Does anti-CTACK antibody A05293 work for IHC with skin of abdomen?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
According to the expression profile of skin of abdomen, CCL27 is highly expressed in skin of abdomen. So, it is likely that anti-CTACK antibody A05293 will work for IHC with skin of abdomen.
Boster Scientific Support
Answered: 2019-02-25
Question
Is this A05293 anti-CTACK antibody reactive to the isotypes of CCL27?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
The immunogen of A05293 anti-CTACK antibody is Synthesized peptide derived from the Internal region of human CTACK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-05
