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Product Info Summary
| SKU: | M00020-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CTLA4 Mouse Monoclonal Antibody [Clone ID: OTI8F1]
SKU/Catalog Number
M00020-2
Size
100 µl
Description
Boster Bio CTLA4 mouse monoclonal antibody,clone OTI8F1. Catalog# M00020-2. Tested in FC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CTLA4 Mouse Monoclonal Antibody [Clone ID: OTI8F1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00020-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8F1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CTLA4 (NP_001032720) produced in HEK293T cell.
Reactive Species
M00020-2 is reactive to CTLA4 in Human
Calculated molecular weight
24.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00020-2 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTLA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTLA4 (1:500).
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Western blot analysis of human peripheral blood mononuclear cells (PBMCs) by using anti-CTLA4 monoclonal antibody. Left: Unstimulated PBMC. Right: PBMC stimulated with 10ug/ml Phytohemagglutinin for 3 days (1:100).
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HEK293T cells transfected with either CTL4 plasmid (red) or empty vector control plasmid (blue).The cells were immunostained by anti-CTLA4 antibody and analyzed by flow cytometry (1:100).
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Flow cytometric analysis of CTLA4 expression on stimulated human peripheral blood mononuclear cells (PBMC). Unstimulated PBMC
Specific Publications For Anti-CTLA4 Mouse Monoclonal Antibody [Clone ID: OTI8F1] (M00020-2)
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