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Product Info Summary
| SKU: | M05147 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-EndoG Monoclonal Antibody [7G1C10]
SKU/Catalog Number
M05147
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-EndoG Monoclonal Antibody [7G1C10] (Catalog # M05147). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
EndoG monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-EndoG Monoclonal Antibody [7G1C10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05147)
Host
Mouse
Contents
EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 7G1C10
Isotype
IgG1
Immunogen
Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
Reactive Species
M05147 is reactive to ENDOG in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
32.6 kDa
Background of ENDOG
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05147 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 2.5 - 5 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 2.5 and (B) 5 μg/mL.
Specific Publications For Anti-EndoG Monoclonal Antibody [7G1C10] (M05147)
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