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- Table of Contents
FGF2 antibody (A00121-3) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IHC analysis of FGF2 using anti-FGF2 antibody (A00121-3).
FGF2 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
FGF2 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (A00121-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-FGF2 Antibody Picoband®
FGF2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
FGF2 antibody was positively detected by WB in:
human K562 whole cell, human U-87MG whole cell, human SK-OV-3 whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FGF2 antibody was positively detected by IHC in:
human gastric cancer tissue, mouse cerebellum tissue, rat cerebellum tissue, human brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FGF2 antibody was positively detected by ICC/IF in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-FGF2 Antibody Picoband®
View all FGF2 Antibodies
View all FGF2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FGF2, which shares 93.3% amino acid (aa) sequence identity with mouse and rat FGF2.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
18 kDa
Calculated Molecular Weight
30.8 kDa
Background of FGF2
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
Cite This Product
Anti-FGF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00121-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Rabbit
93%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Dog
93%
High Homology
Zebrafish
87%
Moderate Homology
Drosophila
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FGF2 antibody
Immunohistochemistry (IHC) protocol for FGF2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FGF2 antibody
Publications
Application:
Species:
Roquin-1 interaction with Regnase-1 inhibits the progression of rheumatoid arthritis via suppressing FGF2 expression and NF-κB pathway
Published: 2025-03-18
Journal: INFLAMMATION RESEARCH
Application: WB
Impact Factor: 5.40
Species: Rat
PMID: 40097661
Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma
Published: 2023-02-14
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 7.20
Species: Mouse
PMID: 36788749
Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa
Published: 2019-10-10
Journal: Acta Biomaterialia
Application: IHC
Impact Factor: 6.64
Species: Rat
PMID: 31606531
Effect of stachydrine hydrochloride to the prostate hyperplasia model in mice
Published: 2018-12-24
Journal: SAUDI JOURNAL OF BIOLOGICAL SCIENCES
Application: IHC
Impact Factor: 3.14
Species: Mouse
PMID: 31049004
The Antimicrobial Peptide Nal-P-113 Exerts a Reparative Effect by Promoting Cell Proliferation, Migration, and Cell Cycle Progression
Published: 2018-09-12
Journal: Biomed Research International
Application: IHC
Impact Factor: 2.58
Species: Rat
PMID: 30298136
MiR-129-5p inhibits non-small cell lung cancer cell stemness and chemoresistance through targeting DLK1
Published: 2017-06-12
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Impact Factor: 2.47
PMID: 28619508
Osteogenesis and mineralization in a rabbit mandibular distraction osteogenesis model is promoted by the human LMP-1 gene
Published: 2015-01-29
Journal: JOURNAL OF ORTHOPAEDIC RESEARCH
Application: WB
Impact Factor: 2.99
Species: Rabbit
PMID: 25641592
Effect of angiogenesis inhibitor SU6668 in combination with 5-Fu on liver metastasis from transplantation tumors of human colorectal cancer in nude mice
Published: 2014-10-15
Journal: International Journal of Clinical and Experimental Medicine
Application: IHC
Impact Factor: 1.42
Species: Mouse
PMID: 25419401
MicroRNA-503 targets FGF2 and VEGFA and inhibits tumor angiogenesis and growth
Published: 2013-01-22
Journal: CANCER LETTERS
Application: WB,IHC
Impact Factor: 4.26
Species: Human,Mouse
PMID: 23352645
Basic fibroblast growth factor stimulates epithelial cell growth and epithelial wound healing in canine corneas
Published: 2009-04-21
Journal: VETERINARY OPHTHALMOLOGY
Impact Factor: 1.56
PMID: 19392876
Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells
Published: 2006-09-04
Journal: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
Impact Factor: 1.07
Species: Pig
PMID: 16864426
Application of bFGF and BDNF to Improve Angiogenesis and Cardiac Function
Published: 2006-08-14
Journal: JOURNAL OF SURGICAL RESEARCH
Application: IHC
Impact Factor: 1.96
Species: Dog
PMID: 16904693
Osteogenesis of freeze-dried cancellous bone allograft loaded with autologous marrow-derived mesenchymal cells
Published: 2002-03-22
Journal: Materials Science & Engineering C-Materials for Biological Applications
Impact Factor: 0.91
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Product Promise
We promise Anti-FGF2 Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00121-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026