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Product Info Summary
| SKU: | M00121-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-FGF2 Mouse Monoclonal Antibody [Clone ID: OTI3D10]
SKU/Catalog Number
M00121-3
Size
100 µl
Description
Boster Bio bFGF (FGF2) mouse monoclonal antibody, clone OTI3D10 (formerly 3D10). Catalog# M00121-3. Tested in FC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-FGF2 Mouse Monoclonal Antibody [Clone ID: OTI3D10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00121-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3D10
Isotype
IgG2b
Immunogen
Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.
Reactive Species
M00121-3 is reactive to FGF2 in Human, Mouse, Rat
Calculated molecular weight
30.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00121-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.
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HEK293T cells transfected with either FGF2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BFGF antibody (M00121-3)
Specific Publications For Anti-FGF2 Mouse Monoclonal Antibody [Clone ID: OTI3D10] (M00121-3)
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