Anti-HARS/Hisrs Antibody

HARS1 antibody

Boster Bio Anti-HARS/Hisrs Antibody catalog # A05242. Tested in IF, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05242
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IF, WB

Product Name

Anti-HARS/Hisrs Antibody

View all HARS1 Antibodies

SKU/Catalog Number

A05242

Size

100μl

Form

Liquid

Description

Boster Bio Anti-HARS/Hisrs Antibody catalog # A05242. Tested in IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HARS/Hisrs Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05242)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from C-terminal of human HARS.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05242 is reactive to HARS in Human, Mouse

Applications

A05242 is guaranteed for IF, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
Immunofluorescence, 1:100-1:500

Validation Images & Assay Conditions

Gene/Protein Information For HARS (Source: Uniprot.org, NCBI)

Uniprot ID

P12081

Gene ID

3035

Gene Name

HARS

Full Name

Histidine--tRNA ligase, cytoplasmic

Weight

57.411kDa

Superfamily

class-II aminoacyl-tRNA synthetase family

Alternative Names

Histidine--tRNA ligase, cytoplasmic HARS1 CMT2W, HARS, HRS, USH3B histidyl-tRNA synthetase 1 histidine--tRNA ligase, cytoplasmic|HisRS|Jo-1 antigen|histidine translase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HARS, check out the HARS Infographic

HARS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HARS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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4 Customer Q&As for Anti-HARS/Hisrs Antibody

Question

Will A05242 anti-HARS/Hisrs antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Dhar

Verified customer

Asked: 2020-03-19

Answer

You can see on the product datasheet, A05242 anti-HARS/Hisrs antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-19

Question

We are currently using anti-HARS/Hisrs antibody A05242 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-20

Answer

The anti-HARS/Hisrs antibody (A05242) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-20

Question

Will anti-HARS/Hisrs antibody A05242 work on monkey WB with brain?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

Our lab technicians have not tested anti-HARS/Hisrs antibody A05242 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-HARS/Hisrs antibody A05242 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-08

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung uterus using anti-HARS/Hisrs antibody A05242. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-29

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-29

Size

Total: $299

SKU:A05242

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A05242
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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