Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M12423 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody
SKU/Catalog Number
M12423
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody (Catalog# M12423). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12423)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
UP-R1
Isotype
Mouse IgG1 kappa
Immunogen
Human CD158f-Ig fusion protein. The mouse monoclonal antibody UP-R1 recognizes an extracellular epitope on CD158f (KIR2DL5), a 60 kDa glycoprotein serving as a HLA class I ligand, and mainly expressed on a subset of NK cells and a small population of T cells. Its expression is highly polymorphic between individuals.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M12423 is reactive to KIR2DL5A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
40.7 kDa
Background of KIR2DL5A
CD158f, also known as KIR2DL5, is a polymorphic 60 kDa transmembrane glycoprotein with two Ig-like extracellular domains by which it recognize HLA class I molecules. Its long intracellular domain contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that upon extracellular ligand-mediated phosphorylation serve as docking sites for inhibitory phosphatases, which results in blocking natural cytotoxicity as well as antibody-dependent cytotoxicity of the particular NK cell, and its adhesion toward target cells. Together with other killer inhibitory receptors CD158f is important for immunological tolerance to discriminate between normal and abnormal cells. Besides NK cells it is expressed on a small population of cytotoxic T cells. Expression of CD158f alleles is highly variable in the population.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12423 is guaranteed for Flow Cytometry, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
Click image to see more details
Flow cytometry surface staining pattern of KIR2DL5A (CD158f) transfected HEK-293 cells co-transfected with YFP coding plasmid using anti-human CD158f (UP-R1) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Click image to see more details
Flow cytometry surface staining patterns of non-transfected HEK-293 cells and HEK-293 cells transfected with KIR-family coding plasmids co-transfected with YFP coding plasmid using anti-human CD158f (UP-R1) purified antibody (concentration in sample 4 µg/ml, GAM APC).
Click image to see more details
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD56 (LT56) APC antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD158f (UP-R1) purified antibody (concentration in sample 3 µg/ml) GAM PE.
Click image to see more details
Separation of human CD158f positive CD56 positive NK cells (red-filled) from CD158f negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD158f (UP-R1) purified antibody (concentration in sample 3 µg/ml) GAM PE.
Click image to see more details
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD158f (UP-R1) purified antibody (concentration in sample 3 µg/ml) GAM PE.
Specific Publications For Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody (M12423)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Hu CD158f Purified KIR2DL5A Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
